Human Pro-IGF-I E antiserum (71 – 86)


The Eagle Bioscience’sHuman Pro-IGF-I E antiserum (71 – 86) is for research use only and not to be used for diagnostic procedures.

Human Pro-IGF-I E antiserum (71 – 86)

The Eagle Bioscience’s Human Pro-IGF-I E antiserum (71 – 86) is for research use only and not to be used for diagnostic procedures.

Product Specifications


Rabbit antiserum


Human ProIGF-I E (71-86) antiserum was prepared by immunising rabbits with a 16 amino acid peptide derived from the E domain of human ProIGF-I E linked to diphtheria toxoid. The peptide sequence is located immediately downstream of the cleavage site at the C terminus of mature IGF-I and as a consequence, the antiserum recognises all ProIGF-I E proteins derived from alternative splicing of the human igf-1 gene. It does not recognise mature IGF-I or IGF-II. It is likely to recognise ProIGF-I E proteins in non-human primates, rabbits and guinea-pigs but not other species due to alternative splicing of the igf-1 gene  

  • Immunogen: Derived from UniProtKB/Swiss-Prot sequence ID P05019 isoform 1 also known as IGF-IB which is recognised as the canonical sequence. The peptide sequence is amino-acids 119-134, derived from ProIGF-I EB, conjugated to diphtheria toxoid.
  • Quantity: 100 µL
  • Specificity: Recognises Human ProIGF-I EB (isoform 1, P05019); ProIGF-I EA (isoform 2, P05019-2); ProIGF-I EC (Q13429). No cross-reactivity against mature IGF-I or IGF-I.
  • Appearance:Lyophilized powder
  • Storage/Stability:   At least 2 years at 2 – 4°C (lyophilized). After reconstitution store at -20°C or -80°C. Avoid freeze-thaw cycles
  • Reconstitution:Dissolve the vial contents in 100 µl sterile water and then dilute in a buffer suitable for the application.
  • Application and Titre:Each application and titre should be determined in house. As a guideline the antiserum can be used at a dilution of 1:1000 for Western immunoblotting.

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Package Inserts

Please note: All documents above are for reference use only and should not be used in place of the documents included with this physical product. If digital copies are needed, please contact us.

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