Human LR3 IGF-I (Media Grade)

$490.00$1,890.00

The Eagle Bioscience’s Human LR3 IGF-I (Media Grade) is for research use only and not to be used for diagnostic procedures.

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Human LR3 IGF-I (Media Grade)

The Eagle Bioscience’s Human LR3 IGF-I (Media Grade) is for research use only and not to be used for diagnostic procedures.


Product Specifications

Source:

IGF-I is produced in E.coli using a patented expression system and the IGF-I is correctly folded and purified by several chromatography steps to yield Receptor Grade IGF-I.

Description:

Human LR3 insulin-like Growth Factor-I (LR3IGF-I) is an 83 amino acid analogue of IGF-I comprising the complete human IGF-I sequence with the substitution of an Arginine for the Glutamine at position 3, plus a 13 amino acid extension peptide at the N-terminus. Human LR3IGF-I is more potent than IGF-I in vitro and in vivo. This increased potency is due to reduced binding of LR3IGF-I to most of the IGF binding proteins which modify the biological actions of IGF-I. Human LR3IGF-I binds to the type 1 IGF receptor with similar affinity to wild type IGF-I.

LR3IGF-I was developed by GroPep specifically for supplementation of mammalian cell culture to support the survival and proliferation of cells. It is engineered to have a higher biological potency than native IGF-I or IGF-II and has several advantages over recombinant insulin. Supplementation of cell cultures with LR3IGF-I at a much lower concentration results in equivalent or better productivity than supplementation with standard concentrations of insulin. LR3IGF-I is better able to stimulate the type I IGF receptor and thus induce a higher level of activation of intracellular signalling molecules which are responsible for promoting cell survival by inhibition of apoptosis. 

  • Purity: >95 % (by SDS gel electrophoresis)
  • Quantity: 1 mg, 10 mg
  • Molecular Weight: 9110.6 ± 2 Daltons – confirmed by Mass Spectrometry
  • N-terminal sequence analysis of recombinant product: Analysis of 18 residues
  • Biological Activity: Proliferation of Chinese Hamster Ovary (CHO) cells (EC50 < 350 ng/ml)
  • Endotoxin (LAL): <0.1 EU/ug
  • Storage/Stability: At least 2 years at 2-8°C (lyophilized)
  • Appearance: White Powder
  • State: Dried from 0.1 M acetic acid under dry nitrogen at a slight vacuum (-25kPa)

Related Products

IGF-I ELISA Assay
Human Arg3 IGF-1 Biotin
Biotinylated Human Des IGF-1

Documents

Product Manual


Please note: All documents above are for reference use only and should not be used in place of the documents included with this physical product. If digital copies are needed, please contact us.

Product Citations