Anti-HAV IgM Antibody ELISA
The Anti-HAV IgM Antibody ELISA is For Research Use Only
Size: 1×96 wells
Incubation Time: 3.5 hours
Sample Type: Serum, Plasma
Sample Size: 100μL
Alternative Names: Hepatitis-A Virus, Anti Hepatitis-A Virus
Manufactured by Mediagnost
The Anti-HAV IgM ELISA Assay Kit is a “class-capture” enzyme immuoassay. Serum or plasma samples are diluted 1:2000 and added to the wells of a microtiter plate, which have been previously coated with an antibody directed against human IgM antibodies (µ-chain specific). Specific antibodies bind to the antibodies present during an incubation step of 1h at 37°C. After washing, the Hepatitis A-virus is added. During the incubation step the HAV binds to the HAV specific IgM antibodies. The conjugate (monoclonal anti-HAV antibody, peroxidase-labelled) is added and incubated again. After a further washing step substrate is added and further incubated for 30 min. at room temperature. The reaction is terminated on addition of stop solution. The absorbance of the coloured reaction product is measured on a microtiter plate reader. The colour intensity of the reaction corresponds to the concentration of antibodies in the sample.
All test kit materials must be stored between 2 – 8°C in the dark. Strips not to be used immediately should be stored airtight in the laminate bag together with the desiccant. The shelf-life of components after opening is not affected, if used appropriately.
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