High Sensitive Irisin ELISA
The High Sensitive Irisin ELISA is For Research Use Only
Size: 1×96 wells
Sensitivity: 1.29 ng/ml
Dynamic Range: 0.1-1000 ng/mL
Incubation Time: 5 hours
Sample Type: Plasma
Sample Size: 50 µL
Alternative Name: Human hs-Irisin ELISA
SAMPLE COLLECTION & STORAGE INFORMATION
The sample collection and storage conditions listed below are intended as general guidelines.
Blood collection- Collect blood samples into tubes containing EDTA. Gently rock the tubes several times immediately to prevent coagulation. Transfer the blood from tubes to centrifuge tubes containing aprotinin (0.6 TIU/ml blood) and gently rock for a few times to inhibit activity of proteinases. Centrifuge the blood at 1600 x g for 15 minutes at 4°C and collect plasma. Plasma can be kept at – 80°C up to 1 month.
This is a Competitive Enzyme Immunoassay for the quantification of Irisin in Serum and plasma samples. This assay employs the competitive enzyme immunoassay technique. A secondary antibody has been pre-coated onto a microtiter plate and nonspecific binding sites were blocked. Fc regions of primary antibodies specific for target peptides can bind to the secondary antibodies on microtiter plate. The Fab regions of primary antibodies are competitively bound by biotinylated peptide and targeted peptides in samples or standards. The biotinylated peptide interacts with streptavidin-horseradish peroxidase (SA-HRP) which catalyzes the substrate solution. The reaction is monitored by a color change which is readable at OD of 450 nm±2 nm. Quantification of unknown samples is achieved by comparing their absorbance with a reference curve prepared with known standards. The intensity of color development is inversely proportional to the amount of Irisin in the samples.