Vitamin B12 Food ELISA Kit
The Vitamin B12 Food ELISA Kit is For Research Use Only
Size: 1×96 wells
Sensitivity: 0.3 ng/mL
Dynamic Range: 0.4 – 40 ng/mL
Incubation Time: 1.5 hours
Sample Type: Food Products, Cell Culture, Tissue
Sample Size: 3-10 g
Vitamin B12 as a trace element belongs to the biologically important chelate formers. The basic unit consists of a corrin ring with cobalt as a central atom. Cobalt is six fold coordinated by four nitrogen atoms, one cyanide and a dimethylbenzimidazol group. Vitamin B12 forms a stable complex, which is absorbed in the lower part of the small intestine, with the so-called intrinsic factor present in the gastric juice. A lack of vitamin B12 can lead among other things to pernicious anemia. This disease is not generated by an insufficient supply of vitamin B12, but by the absence of intrinsic factor. A pernicious anemia can be treated by a high dosage of vitamin B12.
The existing detection procedures are mainly microbiological methods (1), but also HPLC and thin-layer chromatography, all of which are associated with a high amount of time and instrumentation. With the present Eagle Biosciences Vitamin B12 ELISA Assay test kit it is possible, to determine vitamin B12 quantitatively in vitaminated food (2) in a significantly faster way (2.5 to 4 hours inclusive sample pre-treatment) compared with a conventional microbiological assay (24 to 48 hours).