Human LR3 IGF-I ELISA Assay Kit

$620.00$1,160.00

The Eagle Bioscience’s Human LR3 IGF-I ELISA Assay Kit is intended for the quantitative determination of LR3IGF-I in cell culture supernatants.

SKU: BEK-2233 Categories: , ,

Human LR3 IGF-I ELISA Assay Kit

The Eagle Bioscience’s Human LR3 IGF-I ELISA Assay Kit is for research use only


Sizes:

1 Plate Kit (12 x 8 wells), 2 Plate Kit (24 x 8 wells)

Intended Use

The Eagle Bioscience’s Human LR3 IGF-I ELISA Kit has been developed and validated to quantify LR3IGF-I protein in cell culture medium. It is primary use is to measure LR3IGF-I in media and during downstream processing of media following a production cycle and is not intended for other use. For research use only and not to be used for diagnostic procedures.


Assay Background

Human LR3 insulin-like Growth Factor-I (LR3IGF-I) is an 83 amino acid analogue of human IGF-I comprising the complete IGF-I sequence with the substitution of an Arginine for the Glutamine at position 3, plus a 13 amino acid extension peptide at the N-terminus.

Human LR3IGF-I is more potent than IGF-I in vitro and in vivo. This increased potency is due to reduced binding of LR3IGF-I to most of the IGF binding proteins that modify the biological actions of IGF-I. Human LR3IGF-I binds to the type I IGF receptor with similar affinity to wild type IGF-I.

LR3IGF-I was developed by GroPep Bioreagents specifically for supplementation of mammalian cell culture to support the survival and proliferation of cells. It is engineered to have a higher biological potency than native IGF-I or IGF-II and has several advantages over recombinant insulin. Supplementation of cell cultures with LR3IGF-I at a much lower concentration results in equivalent or better productivity than supplementation with standard concentrations of insulin. LR3IGF-I is better able to stimulate the type I IGF receptor and thus induce a higher level of activation of intracellular signalling molecules which are responsible for promoting cell survival by inhibition of apoptosis.

This LR3IGF-I Rapid ELISA kit combines GroPep Bioreagents’ many years of expertise in the field of IGF research and Biosensis’ established Rapid ELISA platform. This collaboration has resulted in the new LR3IGF-I Rapid ELISA kit which provides the sensitive, specific, and reliable quantification of LR3IGF-I protein in less than 3 hours!

The ELISA kit consists of a pre-coated mouse monoclonal anti- LR3IGF-I capture antibody, a biotinylated anti- LR3IGF-I detection antibody and horseradish peroxidase (HRP)-conjugated streptavidin. The addition of a substrate (3,3’,5,5’- tetramethylbenzidine, TMB) yields a colored reaction product which is directly proportional to the concentration of LR3IGF-I present in samples and the supplied protein standard.

The provided Quality Control (QC) sample serves as a positive control with a defined concentration range of LR3IGF-I protein, formulated in a stabilized buffer solution and designed to assure assay performance.


Related Products

Human LR3 IGF-I (Media Grade)
Rodent IGFBP-3 ELISA Assay
Rat IGF-I

Documents

Product Manual


Please note: All documents above are for reference use only and should not be used in place of the documents included with this physical product. If digital copies are needed, please contact us.

Product Citations