Rodent IGFBP-3 ELISA Assay

Additional Information

Assay Background

Growth Hormone, Insulin-like Growth Factors and their binding proteins build up an endocrine system regulating not only longitudinal growth in humans but also influencing a broad variety of other physiological and pathophysiological processes like energy metabolism or tumor growth. Most effects of Growth Hormone (GH) are exerted by Insulin-like Growth Factors (IGF) mainly produced by the liver but also locally by specific tissues. The effects of IGF are also regulated, specific binding proteins (IGFBP 1-7) regulate bioavailability of IGF. After proteolytic cleavage of the binding proteins IGF is set free and able to bind to its receptor. The autophosphorylation of this thyrosine kinase receptor activates intra cellular signalling cascades. Some of these IGFBPs not only regulate the availability of IGF but also exert IGF-independent effects on cell physiology.

IGFBP-3 is the most abundant IGFBP in circulation and therefore of special relevance in regulation of IGF effects. This is reflected by the indicative value of serum IGFBP-3 concentration in diagnostics of growth disturbances. Regulation is effected e.g. through nourishing situation; Different diets for example affect the IGFBP-3 concentration (Bielohuby et al, 2010). IGFBP-3 has also been shown to be able to induce apoptosis, promote tumor growth and inhibit cellular migration and metastasis dependent on tissue and tumor stage.
Been made e.g. also by the nourishing situation, different diets about the IGFBP-3 concentrations affects a regularization.

Assay Principle

The Eagle Biosciences Mouse/Rat IGFBP-3 (m/rIGFBP-3) ELISA Assay Kit is a so-called Sandwich-Assay. It utilizes two specific and high affinity antibodies for this protein. The IGFBP3 in the sample binds to the immobilized first antibody on the microtiter plate. In the following step, the biotinylated and Streptavidin-Peroxidase conjugated second specific anti-mouse IGFBP-3-Antibody binds in turn to the immobilised mIGFBP-3. In the closing substrate reaction the turn of the colour will be high specific catalysed, quantitatively depending on the m/rIGFBP-3-level of the samples.

Typical Standard Curve