Human Nephrin ELISA Kit


The Human Nephrin ELISA Kit is intended for the quantitation of Human NPHS1 concentrations in cell culture supernates, serum and plasma (heparin, EDTA or citrate). The Eagle Biosciences Human Nephrin ELISA Kit is for research use only and not for diagnostic or therapeutic procedures.

SKU: HNE31-K01 Categories: , ,

Human Nephrin ELISA Kit

The Human Nephrin ELISA Kit is For Research Use Only

Size: 1×96 wells
Sensitivity: <50pg/ml
Standard Range: 156pg/ml-10000pg/ml
Incubation Time: 2 hours 40 minutes
Sample Type: Cell culture supernates, serum and plasma(heparin, EDTA or citrate)
Sample Size: 100 µl
Alternative Name: NPHS1

Assay Principle

The Human NPHS1 Pre-Coated ELISA (Enzyme-Linked Immunosorbent Assay) kit is a solid phase immunoassay specially designed to measure Human NPHS1 with a 96-well strip plate that is pre-coated with antibody specific for NPHS1. The detection antibody is a biotinylated antibody specific for NPHS1. The capture antibody is A monoclonal antibody from mouse, the detection antibody is a biotinylated detection polyclonal antibody from goat. The kit contains recombinant Human NPHS1 with immunogen: Expression system for standard: NSO, Immunogen sequence: Q23-S1055. The kit is analytically validated with ready to use reagents. To measure Human NPHS1, add standards and samples to the wells, then add the biotinylated detection antibody. Wash the wells with PBS or TBS buffer, and add Avidin-Biotin-Peroxidase Complex (ABC-HRP). Wash away the unbounded ABC-HRP with PBS or TBS buffer and add TMB. TMB is substrate to HRP and will be catalyzed to produce a blue color product, which changes into yellow after adding acidic stop solution. The density of the yellow product is linearly proportional to Human NPHS1 in the sample. Read the density of the yellow product in each well using a plate reader, and benchmark the sample wells’ readings against the standard curve to determine the concentration of Human NPHS1 in the sample.

Related Products

Human PSP94 ELISA Kit
Human Annexin A1 ELISA Kit
Human TFF1 ELISA Kit

Additional Information

Assay Background

Nephrin is a protein necessary for the proper functioning of the renal filtration barrier. It is mapped to 19q13.12. This gene encodes a member of the immunoglobulin family of cell adhesion molecules that functions in the glomerular filtration barrier in the kidney. The gene is primarily expressed in renal tissues, and the protein is a type-1 transmembrane protein found at the slit diaphragm of glomerular podocytes. The slit diaphragm is thought to function as an ultrafilter to exclude albumin and other plasma macromolecules in the formation of urine. Mutations in this gene result in Finnish-type congenital nephrosis 1, characterized by severe proteinuria and loss of the slit diaphragm and foot processes.

Typical Standard Curve

Human Nephrin ELISA Kit


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