Human MIA ELISA Kit

$495.00

The Human MIA ELISA Kit is intended for the quantitation of Human MIA concentrations in cell culture supernates, serum, plasma (heparin, EDTA) and saliva. The Eagle Biosciences Human MIA ELISA Kit is for research use only and not for diagnostic or therapeutic procedures.

SKU: MIA31-K01 Categories: , ,

Human MIA ELISA Kit

The Human MIA ELISA Kit is For Research Use Only

Size: 1×96 wells
Sensitivity: <10pg/ml
Standard Range: 15.6pg/ml-1000pg/ml
Incubation Time: 2 hours 40 minutes
Sample Type: Cell culture supernates, serum, plasma (heparin, EDTA) and saliva
Sample Size: 100 µl
Alternative Names: Melanoma Inhibiting Activity, Cartilage-derived Retinoic Acid-sensitive Protein, CD-RAP


Assay Principle

The Human MIA Pre-Coated ELISA (Enzyme-Linked Immunosorbent Assay) kit is a solid phase immunoassay specially designed to measure Human MIA with a 96-well strip plate that is pre-coated with antibody specific for MIA. The detection antibody is a biotinylated antibody specific for MIA. The capture antibody is monoclonal antibody from mouse, the detection antibody is polyclonal antibody from goat. The kit contains recombinant Human MIA with immunogen: Expression system for standard: E.coli; Immunogen sequence: G25-131Q. The kit is analytically validated with ready to use reagents. To measure Human MIA, add standards and samples to the wells, then add the biotinylated detection antibody. Wash the wells with PBS or TBS buffer, and add Avidin-Biotin-Peroxidase Complex (ABC-HRP). Wash away the unbounded ABC-HRP with PBS or TBS buffer and add TMB. TMB is substrate to HRP and will be catalyzed to produce a blue color product, which changes into yellow after adding acidic stop solution. The density of the yellow product is linearly proportional to Human MIA in the sample. Read the density of the yellow product in each well using a plate reader, and benchmark the sample wells’ readings against the standard curve to determine the concentration of Human MIA in the sample.


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Additional Information

Assay Background


Melanoma Inhibiting Activity (MIA), also known as cartilage-derived retinoic acid-sensitive protein(CD-RAP), is an approximately 11-15 kDa protein that is expressed as a noncovalent homodimer. MIA is structurally related to OTOR/Otoraplin and MIA-2 in a small family of secreted proteins with one SH3 domain (1-3). And the MIA gene was mapped to 19q13.32-q13.33 by fluorescence in situ hybridization. Besides, it is a marker for malignant melanoma. MIA functions as a chemoattractant for mesenchymal stem cells and enhances their BMP-2 and TGF-beta3 induced differentiation into chondrocytes. MIA-deficient mice exhibit delayed chondrocyte differentiation but enhanced chondrocyte proliferation and cartilage repair.

Typical Standard Curve


Human MIA ELISA Kit

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