Human KLK5 ELISA Kit

$570.00

The Eagle Biosciences Human KLK5 ELISA Kit is intended for the quantitation of Human KLK5 concentrations in cell culture supernates, serum, plasma (heparin, EDTA), human milk and saliva. The Eagle Biosciences Human KLK5 ELISA Kit is for research use only and not for diagnostic or therapeutic procedures.

SKU: LK531-K01 Categories: , ,

Human KLK5 ELISA Kit

The Human KLK5 ELISA Kit is For Research Use Only

Size: 1×96 wells
Sensitivity: <10pg/ml
Standard Range: 78pg/ml-5000pg/ml
Incubation Time: 2 hours 40 minutes
Sample Type: Cell culture supernates, serum, plasma(heparin , EDTA), human milk and saliva
Sample Size: 100 µl
Alternative Names: Kallikrein gene 5, KLK-L2


Assay Principle

The Human KLK5 Pre-Coated ELISA (Enzyme-Linked Immunosorbent Assay) kit is a solid phase immunoassay specially designed to measure Human KLK5 with a 96-well strip plate that is pre-coated with antibody specific for KLK5. The detection antibody is a biotinylated antibody specific for KLK5. The capture antibody is monoclonal antibody from mouse, the detection antibody is polyclonal antibody from goat. The kit contains recombinant Human KLK5 with immunogen: Expression system for standard: NSO; Immunogen sequence: V23-S293. The kit is analytically validated with ready to use reagents. To measure Human KLK5, add standards and samples to the wells, then add the biotinylated detection antibody. Wash the wells with PBS or TBS buffer, and add Avidin-Biotin-Peroxidase Complex (ABC-HRP). Wash away the unbounded ABC-HRP with PBS or TBS buffer and add TMB. TMB is substrate to HRP and will be catalyzed to produce a blue color product, which changes into yellow after adding acidic stop solution. The density of the yellow product is linearly proportional to Human KLK5 in the sample. Read the density of the yellow product in each well using a plate reader, and benchmark the sample wells’ readings against the standard curve to determine the concentration of Human KLK5 in the sample.


Related Products

Human KLK1 ELISA Kit
Human KLK13 ELISA Kit
Human KLK12 ELISA Kit

Additional Information

Assay Background


Kallikrein gene 5(KLK5, also known as KLK-L2), located on chromosome 19q13.4, is one of the newly identified members of the kallikrein gene family, which is a subgroup of the serine protease enzyme family. In normal human tissues, KLK5 is highly expressed in skin, mammary gland and testis. KLK5 has been suggested to regulate cell shedding(desquamation) in conjunction with KLK7 and KLK14, given its ability to degrade proteins which form the extracellular component of cell junctions in the stratum corneum. It is proposed that KLK5 regulates this process since it is able to self-activate in addition to activating KLK7 and KLK14.

Typical Standard Curve


Documents

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