Human Kallikrein-14 ELISA Kit


The Human Kallikrein-14 ELISA Kit is intended for the quantitation of Human KLK14 concentrations in cell culture supernates, cell lysates, serum and plasma (heparin, EDTA). The Eagle Biosciences Human Kallikrein 14 ELISA Kit is for research use only and not for diagnostic or therapeutic procedures.

SKU: K1431-K01 Categories: , ,

Human Kallikrein-14 ELISA Kit

The Human Kallikrein-14 ELISA Kit is For Research Use Only

Size: 1×96 wells
Sensitivity: <2pg/ml
Standard Range: 15.6pg/ml-1000pg/ml
Incubation Time: 2 hours 40 minutes
Sample Type: Cell culture supernates, cell lysates, serum and plasma(heparin, EDTA)
Sample Size: 100 µl
Alternative Names: KLK14

Assay Principle

The Human KLK14 Pre-Coated ELISA (Enzyme-Linked Immunosorbent Assay) kit is a solid phase immunoassay specially designed to measure Human KLK14 with a 96-well strip plate that is pre-coated with antibody specific for KLK14. The detection antibody is a biotinylated antibody specific for KLK14. The capture antibody is monoclonal antibody from mouse, the detection antibody is polyclonal antibody from goat. The kit contains recombinant Human KLK14 with immunogen: Expression system for standard: NSO; Immunogen sequence: Q19-M248. The kit is analytically validated with ready to use reagents. To measure Human KLK14, add standards and samples to the wells, then add the biotinylated detection antibody. Wash the wells with PBS or TBS buffer, and add Avidin-Biotin-Peroxidase Complex (ABC-HRP). Wash away the unbounded ABC-HRP with PBS or TBS buffer and add TMB. TMB is substrate to HRP and will be catalyzed to produce a blue color product, which changes into yellow after adding acidic stop solution. The density of the yellow product is linearly proportional to Human KLK14 in the sample. Read the density of the yellow product in each well using a plate reader, and benchmark the sample wells’ readings against the standard curve to determine the concentration of Human KLK14 in the sample.

Related Products

Human KLK13 ELISA Kit
Human KLK12 ELISA Kit
Human KLK11 ELISA Kit

Additional Information

Assay Background

Kallikrein-14 is a protein that in humans is encoded by the KLK14 gene. Kallikrein-14 belongs to the kallikrein subgroup of serine proteases, which have diverse physiologic functions in many tissues. Kallikrein-14 gene is identified in the kallikrein gene cluster on chromosome 19q13.4. This gene is one of the fifteen kallikrein subfamily members located in a cluster on chromosome 19. Growing evidence suggests that many kallikreins are implicated in carcinogenesis and some have potential as novel cancer and other disease biomarkers. Kallikrein-14 may activate or inactivate the proteinase-activated receptors F2R, F2RL1 and F2RL3 and other kallikreins including KLK1, KLK3, KLK5 and KLK11.

Typical Standard Curve

Human Kallikrein-14 ELISA Kitz


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