Human KLK11 ELISA Kit

$495.00

The Eagle Biosciences Human KLK11 ELISA Kit is intended for the quantitation of Human KLK11 concentrations in cell culture supernates, cell lysates, serum and plasma (heparin, EDTA). The Eagle Biosciences Human KLK11 ELISA Kit is for research use only and not for diagnostic or therapeutic procedures.

SKU: KLK31-K01 Categories: , ,

Human KLK11 ELISA Kit

The Human KLK11 ELISA Kit is For Research Use Only

Size: 1×96 wells
Sensitivity: <10pg/ml
Standard Range: 156pg/ml-10000pg/ml
Incubation Time: 2 hours 40 minutes
Sample Type: cell culture supernates, cell lysates, serum and plasma(heparin, EDTA)
Sample Size: 100 µl
Alternative Name: Kallikrien-11


Assay Principle

The Human KLK11 Pre-Coated ELISA (Enzyme-Linked Immunosorbent Assay) kit is a solid phase immunoassay specially designed to measure Human KLK11 with a 96-well strip plate that is pre-coated with antibody specific for KLK11. The detection antibody is a biotinylated antibody specific for KLK11. The capture antibody is monoclonal antibody from mouse, the detection antibody is polyclonal antibody from goat. The kit contains recombinant Human KLK11 with immunogen: Expression system for standard: NSO, Immunogen sequence: E19-N250. The kit is analytically validated with ready to use reagents. To measure Human KLK11, add standards and samples to the wells, then add the biotinylated detection antibody. Wash the wells with PBS or TBS buffer, and add Avidin-Biotin-Peroxidase Complex (ABC-HRP). Wash away the unbounded ABC-HRP with PBS or TBS buffer and add TMB. TMB is substrate to HRP and will be catalyzed to produce a blue color product, which changes into yellow after adding acidic stop solution. The density of the yellow product is linearly proportional to Human KLK11 in the sample. Read the density of the yellow product in each well using a plate reader, and benchmark the sample wells’ readings against the standard curve to determine the concentration of Human KLK11 in the sample.


Related Products

Human KLK12 ELISA Kit
Human KLK13 ELISA Kit
Human Kallikrein-14 ELISA Kit

Additional Information

Assay Background


Kallikrein-11 is a protein that in humans is encoded by the KLK11 gene. Kallikreins are a subgroup of serine proteases having diverse physiological functions. Growing evidence suggests that many kallikreins are implicated in carcinogenesis and some have potential as novel cancer and other disease biomarkers. This gene is one of the fifteen kallikrein subfamily members located in a cluster on chromosome 19. Alternate splicing of this gene results in two transcript variants encoding two different isoforms which are differentially expressed.

Typical Standard Curve


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