Human KLK12 ELISA Kit


The Human KLK12 ELISA Kit is intended for the quantitation of Human KLK12 concentrations in cell culture supernates, cell lysates, serum and plasma (heparin, EDTA). The Eagle Biosciences Human KLK12 ELISA Kit is for research use only and not for diagnostic or therapeutic procedures.

SKU: KL231-K01 Categories: , ,

Human KLK12 ELISA Kit

The Human KLK12 ELISA Kit is For Research Use Only

Size: 1×96 wells
Sensitivity: <10pg/ml
Standard Range: 156pg/ml-10000pg/ml
Incubation Time: 2 hours 40 minutes
Sample Type: Cell culture supernates, cell lysates, serum and plasma(heparin, EDTA)
Sample Size: 100 µl
Alternative Name: Kallikrien-12

Assay Principle

The Human KLK12 Pre-Coated ELISA (Enzyme-Linked Immunosorbent Assay) kit is a solid phase immunoassay specially designed to measure Human KLK12 with a 96-well strip plate that is pre-coated with antibody specific for KLK12. The detection antibody is a biotinylated antibody specific for KLK12. The capture antibody is monoclonal antibody from mouse, the detection antibody is polyclonal antibody from goat. The kit contains recombinant Human KLK12 with immunogen: NSO, A18-N248. The kit is analytically validated with ready to use reagents. To measure Human KLK12, add standards and samples to the wells, then add the biotinylated detection antibody. Wash the wells with PBS or TBS buffer, and add Avidin-Biotin-Peroxidase Complex (ABC-HRP). Wash away the unbounded ABC-HRP with PBS or TBS buffer and add TMB. TMB is substrate to HRP and will be catalyzed to produce a blue color product, which changes into yellow after adding acidic stop solution. The density of the yellow product is linearly proportional to Human KLK12 in the sample. Read the density of the yellow product in each well using a plate reader, and benchmark the sample wells’ readings against the standard curve to determine the concentration of Human KLK12 in the sample.

Related Products

Human KLK13 ELISA Kit
Human KLK11 ELISA Kit
Human Kallikrein-14 ELISA Kit

Additional Information

Assay Background

Kallikrein-12 is a protein that in humans is encoded by the KLK12 gene. It belongs to the kallikrein subgroup of serine proteases, which have diverse physiologic functions in many tissues. KLK12 is expressed primarily in salivary gland, stomach, uterus, trachea, prostate, thymus, lung, colon, brain, breast, and thyroid gland and at lower levels in testis, pancreas, small intestine, and spinal cord. The expression of KLK12 is downregulated at the mRNA level in breast cancer tissues and is upregulated by steroid hormones in breast and prostate cancer cell lines.

Typical Standard Curve

Human KLK12 ELISA Kit


Product Documents


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