Human C-MET ELISA Kit

$570.00

The Eagle Biosciences Human C-MET/HGFR ELISA Kit is intended for the quantitation of Human MET concentrations in cell culture supernates, cell lysates, serum and plasma (heparin, EDTA). The Eagle Biosciences Human C-MET/HGFR ELISA Kit is for research use only and not for diagnostic or therapeutic procedures.

SKU: HGF31-K01 Categories: , ,

Human C-MET ELISA Kit

The Human C-MET ELISA Kit is For Research Use Only

Size: 1×96 wells
Sensitivity: <5pg/ml
Standard Range: 62.5pg/ml-4000pg/ml
Incubation Time: 2 hours 40 minutes
Sample Type: Cell culture supernates, cell lysates, serum and plasma (heparin, EDTA)
Sample Size: 100 µl
Alternative Names: HGFR, MET, MNNG HOS Transforming gene, hepatocyte growth factor receptor


Assay Principle

The Human MET Pre-Coated ELISA (Enzyme-Linked Immunosorbent Assay) kit is a solid phase immunoassay specially designed to measure Human MET with a 96-well strip plate that is pre-coated with antibody specific for MET. The detection antibody is a biotinylated antibody specific for MET. The capture antibody is monoclonal antibody from mouse, the detection antibody is polyclonal antibody from goat. The kit contains recombinant Human MET with immunogen: Expression system for standard: NSO; Immunogen sequence: E25-R307(alpha)&S308-T932(beta). The kit is analytically validated with ready to use reagents. To measure Human MET, add standards and samples to the wells, then add the biotinylated detection antibody. Wash the wells with PBS or TBS buffer, and add Avidin-Biotin-Peroxidase Complex (ABC-HRP). Wash away the unbounded ABC-HRP with PBS or TBS buffer and add TMB. TMB is substrate to HRP and will be catalyzed to produce a blue color product, which changes into yellow after adding acidic stop solution. The density of the yellow product is linearly proportional to Human MET in the sample. Read the density of the yellow product in each well using a plate reader, and benchmark the sample wells’ readings against the standard curve to determine the concentration of Human MET in the sample.


Related Products

Human ErbB-2 ELISA Kit
Human GDF-15 ELISA Kit
Human Kallikrein 3 ELISA Kit

Additional Information

Assay Background


C-Met (MET or MNNG HOS Transforming gene) is a proto-oncogene that encodes a protein known as hepatocyte growth factor receptor (HGFR). MET proto-oncogene has a total length of 125,982 bp, and it is located in the 7q31 locus of chromosome 7. MET is a membrane receptor that is essential for embryonic development and wound healing. Activation of MET triggers mitogenesis, and morphogenesis.

Typical Standard Curve


Human C-MET ELISA Kit

Documents

Product Manual


 

Please note: All documents above are for reference use only and should not be used in place of the documents included with this physical product. If digital copies are needed, please contact us.

Product Citations