Mouse Thrombopoietin ELISA Kit

$495.00

The Mouse Thrombopoietin ELISA Kit is intended for the quantitation of Mouse TPO concentrations in cell culture supernates, cell lysates, serum and plasma (heparin, EDTA). The Eagle Biosciences Mouse Thrombopoietin ELISA Kit is for research use only and not for diagnostic or therapeutic procedures.

SKU: MTP11-K01 Categories: , ,

Mouse Thrombopoietin ELISA Kit

The Mouse Thrombopoietin ELISA Kit is For Research Use Only

Size: 1×96 wells
Sensitivity: <5pg/ml
Standard Range: 31.2pg/ml-2000pg/ml
Incubation Time: 2 hours 40 minutes
Sample Type: Cell culture supernates, Cell lysates, Serum and Plasma
Sample Size: 100 µl
Alternative Name: TPO


Assay Principle

The Mouse TPO Pre-Coated ELISA (Enzyme-Linked Immunosorbent Assay) kit is a solid phase immunoassay specially designed to measure Mouse TPO with a 96-well strip plate that is pre-coated with antibody specific for TPO. The detection antibody is a biotinylated antibody specific for TPO. The capture antibody is monoclonal antibody from rat, the detection antibody is polyclonal antibody from goat. The kit contains recombinant Mouse TPO with immunogen: Expression system for standard: NSO; Immunogen sequence: S22-T356. The kit is analytically validated with ready to use reagents. To measure Mouse TPO, add standards and samples to the wells, then add the biotinylated detection antibody. Wash the wells with PBS or TBS buffer, and add Avidin-Biotin-Peroxidase Complex (ABC-HRP). Wash away the unbounded ABC-HRP with PBS or TBS buffer and add TMB. TMB is substrate to HRP and will be catalyzed to produce a blue color product, which changes into yellow after adding acidic stop solution. The density of the yellow product is linearly proportional to Mouse TPO in the sample. Read the density of the yellow product in each well using a plate reader, and benchmark the sample wells’ readings against the standard curve to determine the concentration of Mouse TPO in the sample.


Related Products

High Sensitive Anti-TPO ELISA
Mouse LIF ELISA Kit
Mouse Cystatin C ELISA Kit

Additional Information

Assay Background


Thrombopoietin (TPO) is also called megakaryocyte growth and development factor (MGDF). TPO, a lineage-specific cytokine affecting the proliferation and maturation of megakaryocytes from committed progenitor cells, is believed to be the major physiological regulator of circulating platelet levels. Human, dog, and mouse cDNAs for MGDF are highly conserved and encode open reading frames for proteins of 353, 352, and 356 amino acids, respectively, including predicted signal peptides. MGDF is a novel cytokine that regulates megakaryocyte development and is a ligand for the MPL receptor. The human MGDF gene has been mapped to chromosome 3q26.3. The standard product used in this kit is gene recombinant mouse TPO, consisting of 335 amino acids with the molecular mass of 35KDa. As a result of glycosylation, the molecular mass is 75KDa.

Typical Standard Curve


Documents

Product Documents


 

Please note: All documents above are for reference use only and should not be used in place of the documents included with this physical product. If digital copies are needed, please contact us.

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