1-Methylhistamine ELISA Assay Kit
1-Methylhistamine ELISA Assay Kit Developed and Manufactured in Germany by DLD Diagnostika EA208/96
Size: 1×96 wells
Dynamic Range: 0 – 1000 ng/mL; 0 – 8000 nmol/l
Incubation Time: Overnight
Sample Type: Urine
Sample Size: 20 µL
For Research Use Only
1-Methylhistamine: 1 ng / ml = 8.0 nmol / l
Histamine (nomenclature: 2-(4-imidazolyl)-ethylamine) is a natural substance that is widespread in the human and animal organism. It is easily soluble in water and has a basic character. Biochemically, histamine is one of the biogenic amines and is formed from the amino acid histidine. This decarboxylation takes place with the help of the enzyme histidine decarboxylase. The biosynthesis of histamine takes place in the mast cells, cells of the epidermis and gastric mucosa and in the nerve cells.
Histamine can be released in a burst from mast cells and basophilic granulocytes when the storage cells are stimulated with the corresponding allergen. This stimulation occurs through the binding of the allergen to the specific IgE antibodies on the surface of the target cells. However, this effect does not occur upon first contact with an allergen. Initial contact leads to the formation of plasma cells that produce and release specific IgE antibodies. These bind to the corresponding receptors of the mast cells (sensitization). At subsequent exposure to the allergen, the allergen binds directly to the IgE antibodies of the mast cells, triggering the spontaneous release of histamine from the granules of the mast cells (immediate allergic reaction).
Circulating histamine is rapidly converted into 1-methylhistamine (Nmethylhistamine) by histamine-N-methyltransferase. Excretion occurs into the urine and therefore the determination of this metabolite in the urine is of interest.
This assay is for research use only! Not for use in diagnostic procedures!
The 1-Methylhistamine ELISA Kit contains reagents for the quantitative determination of derivatized 1-methylhistamine in human urine samples. After sample preparation in the preparation plate, derivatization is carried out in the ELISA plate. In this process, 1-methylhistamine is quantitatively converted into N-acyl-1-methylhistamine by the acylation reagent. The 1-methylhistamine ELISA is a competitive enzyme immunoassay. Antigen bound to the solid phase and free antigen in solution compete for a defined number of antibody binding sites. When the system is in equilibrium, the unbound antigen-antibody complex is removed in a washing step and the correspondingly bound complex is detected using a peroxidase conjugate and determined via the conversion of tetramethylbenzidine (TMB). The TMB/POD reaction is stopped and measured at 450 nm. The concentration of the antigen-antibody complex bound to the solid phase is inversely proportional to the concentration of the antigen in the sample.