1-Methylhistamine ELISA Assay Kit

$590.00

The Eagle Biosciences 1-Methylhistamine ELISA Assay kit is an enzyme-linked immunosorbent assay used for the quantitative and very sensitive determination of 1-Methylhistamine in biological samples including urine, plasma, and cell culture samples. The 1-Methylhistamine ELISA Assay kit is for research use only and should not be used in diagnostic procedures.

SKU: MHU34-K01 Categories: , ,

1-Methylhistamine ELISA Assay Kit

For Research Use Only

Size: 1×96 wells
Sensitivity: Urine:  2.5 ng/ml, Plasma/Cell Culture:  0.13 ng/ml
Dynamic Range: Urine:  10 -1000 ng/ml, Plasma/Cell Culture:  0.2 – 20 ng/ml
Incubation Time: Overnight
Sample Type: Urine, Plasma, Cell Culture
Sample Size: 20 µL

Controls Included

Product Developed and Manufactured in Germany by DLD Diagnostika EA208/96


Conversion factor:
1-Methylhistamine: 1 ng / ml = 8.0 nmol / l

Additional Information

Assay Background


The Eagle Biosciences 1-Methylhistamine ELISA Assay kit is an Enzyme-linked immunosorbent assay used for the quantitative and very sensitive determination of 1-Methylhistamine in biological samples including urine, plasma, and cell culture samples.  The 1-Methylhistamine ELISA Assay kit is for research use only and should not be used in diagnostic procedures.  Histamine (β-imidazole-ethylamine) a biogenic amine and is a product of the histidine metabolism. It is produced by decarboxylation of histidine.  Histamine is widely distributed in mammalian tissues. It is bound to heparin (as inactive form) and stored in the granules of basophilic leukocytes and mast cells and is actively released as required. These cells, if sensitized by IgE antibodies attached to their membranes, degranulate when exposed to the appropriate antigen.  Histamine plays a major rule in the initial phase of an anaphylactic reaction.  The quantification of histamine in plasma after allergen administration is of clinical interest.

Histamine is part of the immune response to foreign pathogens and it increases the permeability of the capillaries to white blood cells and other proteins, in order to allow them to engage foreign invaders in the affected tissues.  1-Methylhistamine is a histamine metabolite. It is a product of histamine 1- methyltransferase. Urinary histamine and 1-methylhistamine are highly correlated with histamine in plasma. Therefore, allergic reactions can be examined by determination of histamine and 1-methylhistamine in urine.

Assay Principle


The competitive 1-Methylhistamine ELISA kit uses the microtiter plate format. 1-Methylhistamine is bound to the solid phase of the microtiter plate. Acylated 1-methylhistamine and solid phase bound 1¿methylhistamine compete for a fixed number of antiserum binding sites. When the system is in equilibrium, free antigen and free antigen-antiserum complexes are removed by washing. The antibody bound to the solid phase antigen is detected by anti-rabbit/peroxidase. The substrate TMB/peroxidase reaction is monitored at 450 nm. The amount of antibody bound to the solid phase antigen is inversely proportional to the 1-methylhistamine concentration of the sample.

Typical Standard Curve


Urine

Plasma, Cell Culture Samples

Manual

Product Manual