Adiponectin High Sensitive ELISA Assay Kit
Adiponectin High Sensitive ELISA Assay Kit is for Research Use Only
Size: 1 x 96 wells
Sensitivity: 0.06 ng/mL
Dynamic Range: 2–50 ng/mL
Incubation Time: 105 minutes
Sample Type: Human serum or plasma
Sample Size: 20 μL
Controls Included
INTERFERENCE
Interference testing was performed according to CLSI guideline EP7-A2. Serum samples with varying levels of adiponectin were spiked with potential interfering substances at recommended levels and analyzed. Results were compared to the same serum samples with no extra substances added to calculate the % interference. The following substances were tested and did not show significant interference in the assay: hemoglobin up to 0.25 g/L, bilirubin conjugated and free up to 85 μM, triglycerides up to 5.5 mg/mL and human serum albumin up to 60 g/L.
Assay Principle
Adiponectin is a hormone that modulates glucose regulation and fatty acid oxidation. It is secreted from adipose tissue and placenta into the bloodstream and represents 0.01% of all plasma protein. Adiponectin increases insulin sensitivity and decreases plasma glucose by increasing tissue fat oxidation. Adiponectin concentrations correlate negatively with glucose, insulin and triglycerides (TG) concentrations, liver fat content and body mass index and positively with high density lipoprotein cholesterol levels, hepatic insulin sensitivity and insulin stimulated glucose disposal. Adiponectin levels decrease in patients with type 2 diabetes and in patients with coronary heart disease.
Adiponectin could be used as a marker for:
- Energy metabolism and body weight regulation.
- Metabolic syndrome.
- Type 2 diabetes.
- Coronary artery disease.
- Atherosclerosis.
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