FGF-19 ELISA Assay Kit
The FGF-19 ELISA Assay Kit is For Research Use Only
Size: 1×96 wells
Sensitivity: 31.2pg/mL
Standard Range: 31.2pg/mL-2000pg/mL
Incubation Time: 2 hours 35 minutes
Sample Type: Serum, plasma, and cell culture supernates
Sample Size: 100 µl
Alternative Names: Fibroblast growth factor 19
Assay Principle
The FGF-19 assay is a quantitative sandwich ELISA. The immunoplate is pre-coated with a rabbit polyclonal antibody specific for human FGF-19. Standards and samples are pipetted into the wells and any human FGF-19 present is bound by the immobilized antibody. After washing away any unbound substances, a biotin labelled polyclonal antibody specific for human FGF-19 is added to the wells. After wash step to remove any unbound reagents, streptavidin-HRP conjugate (STP-HRP) is added. After the last wash step, an HRP substrate solution is added and color develops in proportion to the amount of human FGF-19 bound initially. The assay is stopped and the optical density of the wells determined using a microplate reader. Since the increases in absorbance are directly proportional to the amount of captured human FGF-19, the unknown sample concentration can be interpolated from a reference curve included in each assay.
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