Rapid Cystatin C ELISA Assay Kit


The Rapid Cystatin C ELISA Assay Kit is designed for quantification of human cystatin c in serum, plasma, and urine samples. The Eagle Biosciences Rapid Cystatin C ELISA Assay Kit is for research use only and not to be used for diagnostic purposes.

SKU: CYS31-K01 Categories: , ,

Rapid Cystatin C ELISA Assay Kit

The Rapid Cystatin C ELISA Assay Kit is For Research Use Only

Size: 1×96 wells
Sensitivity: 0.23ng/mL
Standard Range: 0.23ng/mL-15ng/mL
Incubation Time: 45 minutes
Sample Type: Serum, plasma, and urine
Sample Size: 100 µl
Alternative Names: Cystatin 3

Assay Principle

The Rapid Cystatin C assay is a sandwich ELISA designed for the quantitative detection of human cystatin c in samples in 1 hour. The immunoplate is pre-coated with antibody specific to human cystatin c. Standards and samples are pipetted into the wells and any human cystatin c present is sandwiched by the immobilized antibody and a second horseradish peroxidase (HRP)-linked antibody specific to human cystatin c that is co-incubated with the samples. After wash step to remove any unbound substances, the HRP substrate solution is added and colour develops in proportion to the amount of human cystatin c bound initially. The assay is stopped and the optical density of the wells determined using a micro-plate reader. Since the increases in absorbance are directly proportional to the amount of captured human cystatin c, the unknown sample concentration can be interpolated from a reference curve included in each assay.

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Additional Information

Assay Background

Human cystatin c (or cystatin 3), which is composed of 120 amino acid residues, belongs to the cystatins superfamily that inactivates lysosomal cysteine proteinases. As a strongly cationic and low-molecular weight (13.4 kDa) protein, it is almost freely filtered across the glomerular membrane, and is mainly used as a biomarker of kidney function. A growing body of evidence suggests that cystatin c is a more reliable biomarker of glomerular filtration rate than creatinine. In addition to kidney disease, altered serum levels of cystatin c are associated with several types of cardiovascular disease, including myocardial infarction, stroke, heart failure, peripheral arterial disease and metabolic syndrome. It also seems to play a role in brain disorders involving amyloid, such as Alzheimer’s disease. Furthermore, Cystatin c has also been investigated as a prognostic marker in several forms of cancer.

Typical Standard Curve

Rapid Cystatin C ELISA Kit


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