Dihydrotestosterone (DHT) ELISA Assay Kit

$250.00

The Dihydrotestosterone (DHT) ELISA Assay Kit (enzyme-linked immunoassay kit) is intended for the quantitative determination of Dihydrotestosterone in human serum. The Dihydrotestosterone ELISA Assay Kit is for research use only and not to be used in diagnostic procedures.

SKU: DHT31-K01 Categories: , ,

Dihydrotestosterone (DHT) ELISA Assay Kit

The Dihydrotestosterone (DHT) ELISA Assay Kit is for research use only

Size: 1×96 wells
Sensitivity: 17 pg/mL
Dynamic Range: 25–2500 pg/mL
Incubation Time: 2 hours
Sample Type: Serum
Sample Size: 25 µL
Controls Included


Dihydrotestosterone (DHT) ELISA Background

Dihydrotestosterone (DHT) is the most active natural androgen in humans with a principal role in the development of primary and secondary sexual characteristics and potential participation in a myriad of other physiological processes. The bulk of androgen production takes place mainly in the Leydig cells of the testes. Androgens circulate in the blood bound to proteins, especially sex hormone binding globulin (SHBG) from peripheral conversion of testosterone, while in females most of the DHT is derived from androstenedione.

Some of the main clinical indication of the DHT measurement in serum are investigations of delayed puberty in men and evaluation of the presence of active testicular tissue.

Specimen Collection and Storage

Approximately 0.1 mL of serum is required per duplicate determination. Collect 4–5 mL of blood into an appropriately labeled tube and allow it to clot. Centrifuge and carefully remove the serum layer. Store at room temperature for up to seven days, at 2-8°C for up to fourteen days or freeze at or below -20°C for up to one month. Consider all human specimens as possible biohazardous materials and take appropriate precautions when handling.


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Additional Information

Assay Principle


The DHT ELISA Assay kit is a competitive immunoassay. Competition occurs between DHT present in calibrators, controls and patient samples and an enzyme-labelled antigen
(conjugate) for a limited number of anti-DHT antibody binding sites on the microplate wells. After a washing step that removes unbound materials, the enzyme substrate is added, and 30 minutes later the enzymatic reaction is terminated by addition of stopping solution. The resulting optical density (OD), measured with a microplate reader, is inversely proportional to the concentrations of DHT in the sample. A calibrator curve is plotted with a provided set of calibrators to directly calculate the concentration of DHT in patients samples and controls.

Typical Standard Curve


Documents


Publications

Citations


Cardaci, T.D., Machek, S.B., Wilburn, D.T., et al. High-Load Resistance Exercise Augments Androgen Receptor-DNA Binding and Wnt/b-Catenin Signaling without Increases in Serum/Muscle Androgens or Androgen Receptor Content. Nutrients (2020) https://doi.org/10.3390/nu12123829