Dihydrotestosterone (DHT) ELISA Assay Kit
The Dihydrotestosterone (DHT) ELISA Assay Kit is for research use only
Size: 1×96 wells
Sensitivity: 17 pg/mL
Dynamic Range: 25–2500 pg/mL
Incubation Time: 2 hours
Sample Type: Serum
Sample Size: 25 µL
Dihydrotestosterone (DHT) ELISA Background
Dihydrotestosterone (DHT) is the most active natural androgen in humans with a principal role in the development of primary and secondary sexual characteristics and potential participation in a myriad of other physiological processes. The bulk of androgen production takes place mainly in the Leydig cells of the testes. Androgens circulate in the blood bound to proteins, especially sex hormone binding globulin (SHBG) from peripheral conversion of testosterone, while in females most of the DHT is derived from androstenedione.
Some of the main clinical indication of the DHT measurement in serum are investigations of delayed puberty in men and evaluation of the presence of active testicular tissue.
Specimen Collection and Storage
Approximately 0.1 mL of serum is required per duplicate determination. Collect 4–5 mL of blood into an appropriately labeled tube and allow it to clot. Centrifuge and carefully remove the serum layer. Store at room temperature for up to seven days, at 2-8°C for up to fourteen days or freeze at or below -20°C for up to one month. Consider all human specimens as possible biohazardous materials and take appropriate precautions when handling.