Human IgA ELISA Assay

$365.00

The Eagle Biosciences Human IgA ELISA Assay Kit is a highly sensitive two-site enzyme linked immunoassay (ELISA) for measuring IgA in serum or plasma of Humans. For research use only.

Human IgA ELISA Assay

The Human IgA ELISA Assay is For Research Use Only

Size: 1×96 wells
Sensitivity: 6.477 ng/ml
Dynamic Range: 12.5 ng/ml – 800 ng/ml
Incubation Time: 70 minutes
Sample Type: Milk, Plasma, Serum, Tissue Culture Fluids
Sample Size: 100 μL


Assay Principle

The principle of the double antibody sandwich ELISA is represented in Figure 1. In this assay the IgA present in samples reacts with the anti-IgA antibodies which have been adsorbed to the surface of polystyrene microtitre wells. After the removal of unbound proteins by washing, anti-IgA antibodies conjugated with horseradish peroxidase (HRP), are added. These enzyme-labeled antibodies form complexes with the previously bound IgA. Following another washing step, the enzyme bound to the immunosorbent is assayed by the addition of a chromogenic substrate, 3,3’,5,5’-tetramethylbenzidine (TMB). The quantity of bound enzyme varies directly with the concentration of IgA in the sample tested; thus, the absorbance, at 450 nm, is a measure of the IgA concentration in the test sample. The quantity of IgA in the test sample can be interpolated from the standard curve constructed from the standards, and corrected for sample dilution.


Specimen Collection and Handling
Blood should be collected by venipuncture. The serum should be separated from the cells after clot formation by centrifugation. For plasma samples, blood should be collected into a container with an anticoagulant and then centrifuged. Care should be taken to minimize hemolysis, excessive hemolysis can impact your results. Assay immediately or aliquot and store samples at -20°C. Avoid repeated freeze thaw cycles.


Related Products

Human IgM ELISA Kit
Human IgE ELISA Assay
Human IgD Assay Kit

Additional Information

Typical Standard Curve


Human IgA ELISA Assay

Documents

Product Documents


 

Please note: All documents above are for reference use only and should not be used in place of the documents included with this physical product. If digital copies are needed, please contact us.

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