Normetanephrine & Metanephrine (Plasma) ELISA Assay Kit

$590.00

The Eagle Biosciences Normetanephrine and Metanephrine (Plasma) ELISA Assay Kit is intended for the quantitative determination of normetanephrine and metanephrine in plasma by a microplate enzyme immunoassay (ELISA). The Normetanephrine and Metanephrine (Plasma) ELISA Assay Kit is for research use only and not to be used in clinical, therapeutic or diagnostic procedures.

Normetanephrine & Metanephrine (Plasma) ELISA Assay Kit

For Research Use Only

Size: 2 x 96 wells
Sensitivity: Metanephrine: 7 pg/ml, Normetanephrine: 7 pg/ml
Dynamic Range: Metanephrine: 20 – 3000 pg/ml, Normetanephrine: 20 – 3000 pg/ml
Incubation Time: 4 hours
Sample Type: EDTA Plasma (Also available for urine samples)
Sample Size: 200 µL

Controls Included

Product Developed and Manufactured in Germany by DLD Diagnostika

Additional Information

Assay Background


Normetanephrine and metanephrine are physiologically formed from the catecholamines noradrenaline and adrenaline by the enzyme catechol-O-methyltransferase (COMT). Increased levels of normetanephrine and metanephrine can be found in pheochromocytoma, ganglioneuroma and other neurogenic tumors. The Normetanephrine & Metanephrine (Plasma) ELISA assay kit provides materials for the quantitative measurement of free normetanephrine and metanephrine in EDTA plasma. Normetanephrine and metanephrine are quantitatively acylated to their N-acyl-derivates.

Assay Principle


The competitive Normetanephrine & Metanephrine (Plasma) ELISA Assay Kit uses the microtiter plate format. Metanephrine and normetanephrine, respectively, are bound to the solid phase of the microtiter plate. Acylated nor-/metanephrines from the sample and solid phase bound nor-/metanephrines compete for a fixed number of antiserum binding sites. When the system is in equilibrium, free antigen and free antigen-antiserum complexes are removed by washing. The antibody bound to the solid phase nor-/metanephrine is detected by anti-rabbit IgG / peroxidase. The substrate TMB / peroxidase reaction is monitored at 450 nm. The amount of antibody bound to the solid phase catecholamine is inversely proportional to the catecholamine concentration of the sample.

Manual

Product Manual