BI-CAT Adrenaline & Noradrenaline ELISA Assay Kit
BI-CAT Adrenaline & Noradrenaline ELISA Assay Kit is developed and manufactured in Germany by DLD Diagnostika
Size: 2 x 96 wells (1 plate for Adrenaline, 1 plate for Noradrenaline)
Sensitivity: Adrenaline (urine): 0.08 ng/ml, (plasma): 5 pg/ml, Noradrenaline (urine): 0.67 ng/ml, (plasma): 45 pg/ml
Dynamic Range: Adrenaline: 0.5 – 250 ng/ml, Noradrenaline: 1.5 – 500 ng/ml
Incubation Time: Overnight
Sample Type: Urine, Plasma
Sample Size: 20 µL (urine), 300 µl (plasma)
For Research Use Only
Alternative Names: Epinephrine, Norepinephrine
Controls Included
Reference Values (Normal)
Urine: Adrenaline = <20μg/day, Noradrenaline = < 90μg/day
Plasma: Adrenaline = < 100/pg/mL; Noradrenaline = < 600 pg/mL
It is suggested that each laboratory establish its own normal ranges
Assay Principle
The competitive BI-CAT Adrenaline & Noradrenaline ELISA uses the microtiter plate format. Adrenaline and noradrenaline, respectively, are bound to the solid phase of the microtiter plate. Acylated catecholamine from the sample and solid phase bound catecholamine compete for a fixed number of antiserum binding sites. When the system is in equilibrium, free antigen and free antigen-antiserum complexes are removed by washing. The antibody bound to the solid phase catecholamine is detected by anti-rabbit IgG/peroxidase. The substrate TMB / peroxidase reaction is monitored at 450 nm. The amount of antibody bound to the solid phase catecholamine is inversely proportional to the catecholamine concentration of the sample.
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