Mouse/Rat GH ELISA Assay
The Mouse/Rat GH ELISA Assay is For Research Use Only
Size: 1×96 wells
Sensitivity: 0.04 ng/ml
Incubation Time: 3 hours
Sample Type: Mouse/Rat Serum, Plasma
Sample Size: 20 µL
Alternative Names: Rodent Growth Hormone ELISA, Mouse/Rat Growth Hormone ELISA, Rodent GH ELISA
Manufactured by Mediagnost
The Eagle Biosciences Mouse/Rat GH ELISA Assay Kit is a so-called sandwich-assay. It utilizes two different specific high affinity polyclonal antibodies for this protein. The GH in the samples binds quantitatively to the immobilized antibody. In the following step, the biotinylated antibody in turn binds GH. After washing, a streptavidin-peroxidase-enzyme conjugate will be added, which will bind highly specific to the biotin of the antibody. Subsequently, the peroxidase catalyzes an enzymatic reaction resulting in a blue coloration. The intensity of the blue color depends on the GH content of the sample. The reaction is stopped by the addition of stop solution and color intensity is quantified by measuring the absorption.
Haemolytic conditions have to be avoided.
• Sample transport is recommended chilled e.g. on cooling elements (blue ice) or frozen on dry ice.
• in firmly closable sample vials
• Storage at -20°C: min. 2 years
• Freeze/-thaw cycles: max. 10
It is recommended to store samples chilled as soon as possible.
For any longer time storage the sample has to be kept frozen at -20°C.