HAV Antigen ELISA Assay
The HAV Antigen ELISA Assay is For Research Use Only
Size: 1×96 wells
Incubation Time: 4.5 hours
Sample Type: Stool, Cell Culture
Sample Size: 50 uL
Sensitivity: ELISA-negative samples nevertheless can contain HAV particles.
Specificity: 91% of the elisa-positive samples were also HAV-PCR positive. Cross reactivity is not known.
cut-off: 16% CV intra-assay
positive control: 3.5% CV intra-assay
Manufactured by Mediagnost
The Eagle Biosciences specimens are pipetted into wells of a microtiter plate previously coated with antibodies directed against HAV. The HAV antigen binds to the fixed antibody and after the incubation period of two hours at 37°C the plate is washed thoroughly.Bound HAV antigen is identified by conjugate addition (monoclonal anti-HAV, peroxidase conjugated) incubated for another two hours at 37°C. Excess conjugate is removed by washing and the substrate is added. After 30 minutes incubation at room temperature the reaction is terminated by adding stop solution. The blue colour of a positive reaction turns to yellow and is measured in a microplate reader at 450 nm. The intensity of the colour indicates the concentration of bound HAV antigen. A positive reaction must be confirmed by neutralising with anti-HAV serum to discriminate false positive reactions which sometimes occurs in stool.
All materials must be stored at 2-8 °C in the dark. Unused microtiter plate strips have to be stored airtight together with the desiccant bag at 2-8°C. The shelf-life of the components after opening is not affected, if used appropriately.
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