Anti-Myelin Auto Antibodies ELISA
The Anti-Myelin Auto Antibodies ELISA is For Research Use Only
Size: 1×96 wells
Sensitivity: 0.014 ng/ml
Incubation Time: 4.75 hours
Sample Type: Serum, Cerebrospinal fluid
Sample Size: 10 µL
Product manufactured by Mediagnost
Myelin-specific proteins have been purified from bovine brain and used for coating of microtiter plates: Myelin-oligodendrocyte glycoprotein (MOG); Myelin Basic Protein (MBP); Myelin-associated Glycoprotein (MAG); Proteolipoprotein (PLP); Alpha-B-Crystallin (CRY). The antibodies in the samples bind specifically to the immobilized antigens. IgG- or IgM specific biotinylated antibodies together with a streptavidin-peroxidase conjugate are employed for the detection of the bound antibodies of the sample. A chemical reaction catalyzed by the peroxidase results in a colour change of the substrate, which is stopped after a defined period of time. The colour intensity correlates positively with the amount of specific antibodies bound. To assess the quantity in each run a ready-to-use diluted human serum is used for calibration. The amount of specific antibody in the standard has been determined by titration. Thus, this assay quantifies the specific antibodies in the sample in arbitrary Mediagnost Units.
Specimen: Storage, Preparation, Transport
Non hemolytic, non icteric and non lipemic serum and cerebrospinal fluid samples can be used in this assay.
Specimen transport within 3 days can be done at room temperature: 20-25°C
Generally, specimens should be cooled as soon as possible. For longterm storage the samples should stored at -20C or below. Repeated freeze/thawing cycles have to be avoided.
Usually a dilution of 1:1000-2000 in dilution buffer VP is sufficient for serum samples. In case of cerebrospinal fluid a dilution of 1:2.5 –1:10 is recommended.