Anti-Myelin Auto Antibodies ELISA

$1,710.00

The Anti-Myelin Auto-Antibodies ELISA Assay Kit allows the quantitative detection of antigen specific IgG and IgM antibodies in serum samples and cerebrospinal fluid. The Anti-Myelin Auto-Antibodies ELISA Assay Kit is for research use only.


Anti-Myelin Auto Antibodies ELISA

The Anti-Myelin Auto Antibodies ELISA is For Research Use Only

Size: 1×96 wells
Sensitivity: 0.014 ng/ml
Incubation Time: 4.75 hours
Sample Type: Serum, Cerebrospinal fluid
Sample Size: 10 µL

Product manufactured by Mediagnost


Assay Principle

Myelin-specific proteins have been purified from bovine brain and used for coating of microtiter plates: Myelin-oligodendrocyte glycoprotein (MOG); Myelin Basic Protein (MBP); Myelin-associated Glycoprotein (MAG); Proteolipoprotein (PLP); Alpha-B-Crystallin (CRY).  The antibodies in the samples bind specifically to the immobilized antigens. IgG- or IgM specific biotinylated antibodies together with a streptavidin-peroxidase conjugate are employed for the detection of the bound antibodies of the sample. A chemical reaction catalyzed by the peroxidase results in a colour change of the substrate, which is stopped after a defined period of time. The colour intensity correlates positively with the amount of specific antibodies bound.  To assess the quantity in each run a ready-to-use diluted human serum is used for calibration. The amount of specific antibody in the standard has been determined by titration. Thus, this assay quantifies the specific antibodies in the sample in arbitrary Mediagnost Units.


Specimen: Storage, Preparation, Transport
Non hemolytic, non icteric and non lipemic serum and cerebrospinal fluid samples can be used in this assay.
Specimen transport within 3 days can be done at room temperature: 20-25°C
Generally, specimens should be cooled as soon as possible. For longterm storage the samples should stored at -20C or below. Repeated freeze/thawing cycles have to be avoided.
Usually a dilution of 1:1000-2000 in dilution buffer VP is sufficient for serum samples. In case of cerebrospinal fluid a dilution of 1:2.5 –1:10 is recommended.


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Additional Information

Assay Background


Several neurodegenerative diseases are at least partially caused by autoimmune reactions. Inflammatory processes result in neuronal loss. Mostly, T-cell dependent immunity is in the central part of the pathological concept. But it is known that in some neurological diseases like multiple sclerosis B-cell dependent immunity is also of relevance in disease progress.  Thus, antibodies are a well-established means in differential diagnostics of neuronal diseases e.g. by the detection of oligoclonal bands or measurement of specific antibodies against neuronal Proteins like aquaporin IV.   This assay allows the quantitative detection of antigen-specific IgG and IgM antibodies in serum samples and cerebrospinal fluid.

Manual

Product Manual