Androstenedione ELISA Assay Kit

$230.00

The Eagle Biosciences Androstenedione ELISA Assay Kit is a competitive immunoenzymatic colorimetric method for quantitative determination of Androstenedione concentration in human serum or plasma. The Androstenedione ELISA Assay Kit is intended for research use only and not to be used in diagnostic procedures.

SKU: DCM008 Categories: , ,

Androstenedione ELISA Assay Kit

For Research Use Only

Size: 1×96 wells
Sensitivity: 0.01 ng/m
Dynamic Range: 0.1 – 10 ng/ml
Incubation Time: 1.5 hour
Sample Type: Serum, Plasma
Sample Size: 25 µL

Controls Included

Product Developed and Manufactured in Italy by Diametra

Additional Information

Assay Background

Androstenedione (also known as Δ4-androstenedione) is a steroid hormone produced in the adrenal glands and the gonads as an intermediate step in the biochemical pathway that produces the androgen testosterone and the estrogens estrone and estradiol. It is the common precursor of male and female sex hormones. Some androstenedione is also secreted into the plasma, and may be converted in peripheral tissues to testosterone and estrogens.

Androstenedione has relatively weak androgenic activity, estimated at ~ 20% of testosterone. However, serum androstenedione levels often exceed testosterone in both normal and disease states. Secretion and production rates also exceed those of testosterone in women in whom significant extra-adrenal conversion of androstenedione to testosterone occurs.

In premenopausal women the adrenal glands and ovaries each produce about half of the total androstendione (about 3 mg/day). After menopause androstenedione production is about halved, primarily due to the reduction of steroid secreted by the ovary. Nevertheless, androstenedione is the principal steroid produced by the postmenopausal ovary. Measurement of serum androstenedione provides a useful marker of androgen biosynthesis. Elevated androstenedione levels have been demonstrated in virilizing congenital adrenal hyperplasia. Serum androstenedione levels are also increased in polycystic ovary syndrome, and in case of hirsutism in women. Elevated serum androstenedione levels may also occur in adrenal and ovarian virilizing tumors.

Assay Principle

In the Androstenedione ELISA Assay Kit, the (antigen) in the sample competes with the antigenic Androstenedione conjugated with horseradish peroxidase (HRP) for binding to the limited number of antibodies anti Androstenedione coated on the microplate (solid phase). After incubation, the bound/free separation is performed by a simple solid-phase washing. Then, the enzyme HRP in the bound-fraction reacts with the Substrate (H2O2) and the TMB Substrate and develops a blue color that changes into yellow when the Stop Solution (H2SO4) is added. The color intensity is inversely proportional to the Androstenedione concentration of in the sample. Androstenedione concentration in the sample is calculated through a calibration curve.

Manual

Product Manual


Publications

Publications

Campen Ka et al. (2018). The Impact of Besphenol S on Bovine Ganulose and Theca Cells. Reproduction in Domestic Animals.  53:450-457.