Androstenedione ELISA Assay Kit


The Eagle Biosciences Androstenedione ELISA Assay Kit is intended for the direct quantitative determination of Androstenedione in human serum by an enzyme immunoassay.

Androstenedione ELISA Assay Kit

For Research Use Only

Size: 1×96 wells
Sensitivity: 0.04 ng/mL
Dynamic Range: 0.1–10 ng/mL
Incubation Time: 80 minutes
Sample Type: Serum
Sample Size: 25 µL

Additional Information

Assay Background

Androstenedione is produced by the adrenals and gonads. As a result, the determination of the level of androstenedione in serum is important in the evaluation of the functional state of these glands. Androstenedione is a precursor of testosterone and estrone. Besides the adrenals, in females, the ovaries have been shown to be an important source of androstenedione. It has been reported that there is a fluctuation day by day of androstenedione during the ovulatory cycle. The principle production of testosterone in females is from the conversion of other related androgens, especially androstenedione. An abnormal testosterone level in women should be accompanied by the estimation of serum androstenedione. The use of serum testosterone determination in conjunction with the enzyme immunoassay of androstenedione can be used to determine if the source of the excess androgen production is adrenal or ovarian.

Assay Principle

The principle of the following enzyme immunoassay test follows the typical competitive binding scenario. Competition occurs between an unlabelled antigen (present in standards, controls and patient samples) and an enzyme-labelled antigen (conjugate) for a limited number of antibody binding sites on the microplate. The washing and decanting procedures remove unbound materials. After the washing step, the enzyme substrate is added. The enzymatic reaction is terminated by addition of the stopping solution. The absorbance is measured on a microtiter plate reader. The intensity of the colour formed is inversely proportional to the concentration of androstenedione in the sample. A set of standards is used to plot a standard curve from which the amount of androstenedione in patient samples and controls can be directly read.


Product Manual



1. Perel E, et al. The Conversion of Androstenedione to Estrone, Estradiol, and Testosterone in Breast Tissue. J Steroid Biochem. 1980; 13:89.
2. Bermúdez JA, et al. Stereochemical Approach to Increase the Specificity of Steroid Antibodies. J Steroid Biochem. 1975; 6:283.
3. Hampl R, et al. The Use of Iodinated Steroid as Radioligand for Testosterone Radio-immunoassay. J Steroid Biochem. 1978; 9:771.
4. Hummer L, et al. An Easy and Reliable Radioimmunoassay of Serum Androstenedione: Age-Related Normal Values in 252 Females Aged 2 to 70 Years. Scand J Clin Lab Invest. 1983; 43:301.
5. Hennam JF, et al. A Cost Reductive Method for the Radioimmunoassay of Plasma Androstenedione. A Comparison of Antisera and the Effects of Chromatography. Acta Endocrinol. 1974; 76:597.
6. Judd HL, et al. Serum Androstenedione and Testosterone Levels During the Menstrual Cycle. J Clin Endo Metabo. 1973; 36:475.
7. Lejeune-Lenair C, et al. A Direct Radio-Immunoassay for Plasma Delta 4-Androstenedione. Application to Children. Clin Chem Acta. 1979; 94:327.
8. Parker LN, et al. An Improved Radioimmunoassay for 4-Androstene-3, 17-Dione. Steroids. 1977; 29:715.
9. Pizarro MA, et al. Human Testicular Metabolism of Testosterone and Androstenedione in Normal and Infertile Men. J Steroid Biochem. 1980; 12:509.
10. Rao PN, et al. Specific Antisera Suitable for Solid-Phase Radioimmunoassay of 11Beta-Hydroxyandrost-4-Ene-3,17-Dione. Steroids. 1974; 24:793.
11. Putz Z, et al. A Selective Radioimmunoassay of Androstenedione in Plasma and Saliva. J Clin Chem Clin Biochem. 1982; 20:761.
12. Schanbacher BD, et al. Simultaneous Determination of Testosterone, 5Alpha-Androstan-17Beta-Ol-3-One, 5Alpha-Androstane-3Alpha, 17Beta-Diol and 5Alpha-Androstane-3Beta, 17Beta-Diol in Plasma of Adult Male Rabbits by Radioimmunoassay(1). Endocrinology. 1975; 97:787.
13. Slaats EH, et al. Interference of Sex-Hormone Binding Globulin in the “Coat-A-Count” Testosterone No-Extraction Radioimmunoassay. Clin Chem. 1987; 33:300.
14. Swinkles LM, et al. Salivary and Plasma Free Testosterone and Androstenedione Levels in Women Using Oral Contraceptives Containing Desogestrel or Levonorgestrel. Ann Clin Biochem. 1988; 23: 354.
15. Thorneycroft IH, et al. A Radioimmunoassay of Androstenedione. Steroids. 1973; 21:111.
16. Check JH, et al. Falsely Elevated Steroidal Assay Levels Related to Heterophile Antibodies Against Various Animal Species. Gynecol Obstet Invest. 1995; 40(2):139–40.