Androstenedione ELISA Assay Kit

$230.00

The Eagle Biosciences Androstenedione ELISA Assay Kit is intended for the direct quantitative determination of Androstenedione in human serum by an enzyme immunoassay.

SKU: ASD31-K01 Categories: , ,

Androstenedione ELISA Assay Kit

For Research Use Only

Size: 1×96 wells
Sensitivity: 0.04 ng/mL
Dynamic Range: 0.1–10 ng/mL
Incubation Time: 80 minutes
Sample Type: Serum
Sample Size: 25 µL

Controls Included

Additional Information

Assay Background

Androstenedione is produced by the adrenals and gonads. As a result, the determination of the level of androstenedione in serum is important in the evaluation of the functional state of these glands. Androstenedione is a precursor of testosterone and estrone. Besides the adrenals, in females, the ovaries have been shown to be an important source of androstenedione. It has been reported that there is a fluctuation day by day of androstenedione during the ovulatory cycle. The principle production of testosterone in females is from the conversion of other related androgens, especially androstenedione. An abnormal testosterone level in women should be accompanied by the estimation of serum androstenedione. The use of serum testosterone determination in conjunction with the enzyme immunoassay of androstenedione can be used to determine if the source of the excess androgen production is adrenal or ovarian.

Assay Principle

The principle of the following enzyme immunoassay test follows the typical competitive binding scenario. Competition occurs between an unlabelled antigen (present in standards, controls and patient samples) and an enzyme-labelled antigen (conjugate) for a limited number of antibody binding sites on the microplate. The washing and decanting procedures remove unbound materials. After the washing step, the enzyme substrate is added. The enzymatic reaction is terminated by addition of the stopping solution. The absorbance is measured on a microtiter plate reader. The intensity of the colour formed is inversely proportional to the concentration of androstenedione in the sample. A set of standards is used to plot a standard curve from which the amount of androstenedione in patient samples and controls can be directly read.

Typical Standard Curve

Manual

Product Manual


Publications

References

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