Adiponectin High Sensitive ELISA Assay Kit

$440.00

The Adiponectin High Sensitive ELISA is for the quantitative determination of Adiponectin in human serum and plasma by an enzyme immunoassay method.

SKU: ADP31-K01 Categories: ,

Adiponectin High Sensitive ELISA Assay Kit

Adiponectin High Sensitive ELISA Assay Kit is for Research Use Only

Size: 1 x 96 wells
Sensitivity: 0.06 ng/mL
Dynamic Range: 2–50 ng/mL
Incubation Time: 105 minutes
Sample Type: Human serum or plasma
Sample Size: 20 μL
Controls Included


INTERFERENCE
Interference testing was performed according to CLSI guideline EP7-A2. Serum samples with varying levels of adiponectin were spiked with potential interfering substances at recommended levels and analyzed. Results were compared to the same serum samples with no extra substances added to calculate the % interference. The following substances were tested and did not show significant interference in the assay: hemoglobin up to 0.25 g/L, bilirubin conjugated and free up to 85 μM, triglycerides up to 5.5 mg/mL and human serum albumin up to 60 g/L.


Assay Principle

Adiponectin is a hormone that modulates glucose regulation and fatty acid oxidation. It is secreted from adipose tissue and placenta into the bloodstream and represents 0.01% of all plasma protein. Adiponectin increases insulin sensitivity and decreases plasma glucose by increasing tissue fat oxidation. Adiponectin concentrations correlate negatively with glucose, insulin and triglycerides (TG) concentrations, liver fat content and body mass index and positively with high density lipoprotein cholesterol levels, hepatic insulin sensitivity and insulin stimulated glucose disposal. Adiponectin levels decrease in patients with type 2 diabetes and in patients with coronary heart disease.
Adiponectin could be used as a marker for:

  • Energy metabolism and body weight regulation.
  • Metabolic syndrome.
  • Type 2 diabetes.
  • Coronary artery disease.
  • Atherosclerosis.

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Additional Information

Assay Background


Adiponectin is a hormone that modulates glucose regulation and fatty acid oxidation. It is secreted from adipose tissue and placenta into the bloodstream and represents 0.01% of all plasma protein. Adiponectin increases insulin sensitivity and decreases plasma glucose by increasing tissue fat oxidation. Adiponectin concentrations correlate negatively with glucose, insulin and triglycerides (TG) concentrations, liver fat content and body mass index and positively with high density lipoprotein cholesterol levels, hepatic insulin sensitivity and insulin stimulated glucose disposal. Adiponectin levels decrease in patients with type 2 diabetes and in patients with coronary heart disease.

Assay Principle


The principle of the adiponectin ELISA is a two-step sandwich enzyme immunoassay. The assay makes use of two highly specific monoclonal antibodies: A monoclonal antibody specifi c for adiponectin is immobilized onto the microplate and another monoclonal antibody specific for a different epitope of adiponectin is conjugated to biotin. During the first step, adiponectin present in the samples and standards is bound to the immobilized antibody and to the biotinylated antibody, thus forming a sandwich complex. Unbound biotinylated antibody is removed by a washing. In the second step, streptavidin-HRP is added, which binds specifi cally to bound biotinylated antibody. Unbound streptavidin-HRP is removed by washing. Next, the enzyme substrate (TMB) is added. The colour intensity of the enzymatic reaction is directly proportional to the concentration of adiponectin. The enzymatic reaction is terminated by the addition of stopping solution. The absorbance is measured on a microplate reader at 450 nm. The concentration of adiponectin in samples and controls can be calculated from of a plot of the standard curve, either graphically or by using immunoassay software.

Typical Standard Curve


Manual

Product Manual