Human LR3 IGF-I ELISA Assay Kit
The Eagle Bioscience’s Human LR3 IGF-I ELISA Assay Kit is for research use only
Sizes:
1 Plate Kit (12 x 8 wells), 2 Plate Kit (24 x 8 wells)
Intended Use
The Eagle Bioscience’s Human LR3 IGF-I ELISA Kit has been developed and validated to quantify LR3IGF-I protein in cell culture medium. It is primary use is to measure LR3IGF-I in media and during downstream processing of media following a production cycle and is not intended for other use. For research use only and not to be used for diagnostic procedures.
Assay Background
Human LR3 insulin-like Growth Factor-I (LR3IGF-I) is an 83 amino acid analogue of human IGF-I comprising the complete IGF-I sequence with the substitution of an Arginine for the Glutamine at position 3, plus a 13 amino acid extension peptide at the N-terminus.
Human LR3IGF-I is more potent than IGF-I in vitro and in vivo. This increased potency is due to reduced binding of LR3IGF-I to most of the IGF binding proteins that modify the biological actions of IGF-I. Human LR3IGF-I binds to the type I IGF receptor with similar affinity to wild type IGF-I.
LR3IGF-I was developed by GroPep Bioreagents specifically for supplementation of mammalian cell culture to support the survival and proliferation of cells. It is engineered to have a higher biological potency than native IGF-I or IGF-II and has several advantages over recombinant insulin. Supplementation of cell cultures with LR3IGF-I at a much lower concentration results in equivalent or better productivity than supplementation with standard concentrations of insulin. LR3IGF-I is better able to stimulate the type I IGF receptor and thus induce a higher level of activation of intracellular signalling molecules which are responsible for promoting cell survival by inhibition of apoptosis.
This LR3IGF-I Rapid ELISA kit combines GroPep Bioreagents’ many years of expertise in the field of IGF research and Biosensis’ established Rapid ELISA platform. This collaboration has resulted in the new LR3IGF-I Rapid ELISA kit which provides the sensitive, specific, and reliable quantification of LR3IGF-I protein in less than 3 hours!
The ELISA kit consists of a pre-coated mouse monoclonal anti- LR3IGF-I capture antibody, a biotinylated anti- LR3IGF-I detection antibody and horseradish peroxidase (HRP)-conjugated streptavidin. The addition of a substrate (3,3’,5,5’- tetramethylbenzidine, TMB) yields a colored reaction product which is directly proportional to the concentration of LR3IGF-I present in samples and the supplied protein standard.
The provided Quality Control (QC) sample serves as a positive control with a defined concentration range of LR3IGF-I protein, formulated in a stabilized buffer solution and designed to assure assay performance.
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