Cardiovascular and Oxidative Stress Assay Kit

Mouse FLT1/VEGFR1 ELISA Kit

$585.00

The Mouse FLT1/VEGFR1 ELISA Kit is intended for the quantitation of Mouse FLT1 concentrations in cell culture supernates and serum. The Eagle Biosciences Mouse FLT1 ELISA Kit is for research use only and not for diagnostic or therapeutic procedures.

SKU: FLT11-K01 Categories: , ,

Mouse FLT1/VEGFR1 ELISA Kit

The Mouse FLT1/VEGFR1 ELISA Kit is For Research Use Only

Size: 1×96 wells
Sensitivity: <10pg/ml
Standard Range: 156pg/ml-10000pg/ml
Incubation Time: 2 hours 40 minutes
Sample Type: Cell culture supernates and Serum
Sample Size: 100 µl
Alternative Names: Vascular endothelial growth factor receptor 1,

Assay Principle

The Mouse Flt1 Pre-Coated ELISA (Enzyme-Linked Immunosorbent Assay) kit is a solid phase immunoassay specially designed to measure Mouse Flt1 with a 96-well strip plate that is pre-coated with antibody specific for Flt1. The detection antibody is a biotinylated antibody specific for Flt1. The capture antibody is monoclonal antibody from rat, the detection antibody is polyclonal antibody from goat. The kit contains recombinant Mouse Flt1 with immunogen: Expression system for standard: NSO, Immunogen sequence: Y23-E759. The kit is analytically validated with ready to use reagents. To measure Mouse Flt1, add standards and samples to the wells, then add the biotinylated detection antibody. Wash the wells with PBS or TBS buffer, and add Avidin-Biotin-Peroxidase Complex (ABC-HRP). Wash away the unbounded ABC-HRP with PBS or TBS buffer and add TMB. TMB is substrate to HRP and will be catalyzed to produce a blue color product, which changes into yellow after adding acidic stop solution. The density of the yellow product is linearly proportional to Mouse Flt1 in the sample. Read the density of the yellow product in each well using a plate reader, and benchmark the sample wells’ readings against the standard curve to determine the concentration of Mouse Flt1 in the sample.

Related Products

Mouse VEGFR2/KDR ELISA Kit
Mouse FLT4/VEGFR3 ELISA Kit
Mouse VEGF ELISA Assay Kit

Additional Information

Assay Background

Vascular endothelial growth factor receptor 1 is a protein that in humans is encoded by the FLT1 gene. Oncogene FLT belongs to the src gene family and is related to oncogene ROS. Like other members of this family, it shows tyrosine protein kinase activity that is important for the control of cell proliferation and differentiation. This protein binds to VEGFR-A, VEGFR-B and placental growth factor and plays an important role in angiogenesis and vasculogenesis. Expression of this receptor is found in vascular endothelial cells, placental trophoblast cells and peripheral blood monocytes. Multiple transcript variants encoding different isoforms have been found for this gene. Isoforms include a full-length transmembrane receptor isoform and shortened, soluble isoforms. The soluble isoforms are associated with the onset of pre-eclampsia.

Typical Standard Curve

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