CLEIA Archives

The Eagle Biosciences MedFrontier FGF23 (Human Intact FGF23) CLEIA ELISA Assay was used in a recently published study looking at the association between FGF23 levels and major adverse cardiovascular events (MACE) and mortality in patients with type 2 diabetes and normal or mildly impaired kidney function.

FGF23 is an endocrine hormone involved in vitamin D metabolism and phosphorous level regulation. Previous studies have shown a link between insulin resistance and an increase in FGF23, which researchers believe affects renal phosphorous handling and leads to a higher risk of MACE.

The study did find evidence of a connection with FGF23 levels and an increased risk of MACE or mortality in participating patients.

ELISA vs. CLEIA

ELISAs use antibodies to detect the presence of a protein in a liquid sample. In the most common form, antigens are applied to a stable surface (usually a microtiter plate). Then the corresponding antibody, linked with an enzyme, is added to form a complex. Finally, the enzyme’s substrate is added, which produces a measurable reaction, usually in the form of a color change.

CLEIAs also utilize antibody-enzyme reactions, but offer a higher level of sensitivity due use of chemiluminescence rather than colorimetric absorbance or fluorescence. Chemiluminescence allows detection of trace amounts of analytes because samples typically have little to no background interference. These reactions are also easily measured using a photomultiplier or photodiode. These types of kits are increasing in popularity due to their increased sensitivity, specificity, reliability, and reproducibility.

Our available CLEIA/CLIA kits

GFAP (Glial Fibrillary Acidic Protein) CLIA Assay Kit
MedFrontier FGF23 (Human Intact FGF23) CLEIA ELISA Assay

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Eagle Biosciences Human Intact FGF23 CLEIA ELISA Used in Diabetes Study

EagleBio Highlights CLEIA Kits

ELISA vs. CLEIA

Our available CLEIA/CLIA kits