Rat Antithrombin III ELISA


The Rat Antithrombin III ELISA Assay Kit is a highly sensitive two-site enzyme linked immunoassay (ELISA) for measuring Antithrombin III in biological samples of rats.

Rat Antithrombin III ELISA

The Rat Antithrombin III ELISA is For Research Use Only

Size: 1×96 wells
Sensitivity: 1.399 ng/ml
Dynamic Range: 12.5 ng/ml – 400 ng/ml
Incubation Time: 100 minutes
Sample Type: Plasma, Serum, Urine
Sample Size: 100 μL
Alternative Names: AT3, AT 3, AT III

Assay Principle

In this Rat Antithrombin III ELISA Assay, the Antithrombin III present in samples reacts with the anti-Antithrombin III antibodies which have been adsorbed to the surface of polystyrene microtitre wells. After the removal of unbound proteins by washing, anti-AT3 antibodies conjugated with horseradish peroxidase (HRP), are added. These enzyme-labeled antibodies form complexes with the previously bound AT3. Following another washing step, the enzyme bound to the immunosorbent is assayed by the addition of a chromogenic substrate, 3,3’,5,5’-tetramethylbenzidine (TMB). The quantity of bound enzyme varies directly with the concentration of AT3 in the sample tested; thus, the absorbance, at 450 nm, is a measure of the concentration of AT3 in the test sample. The quantity of AT3 in the test sample can be interpolated from the standard curve constructed from the standards, and corrected for sample dilution.

Blood should be collected by venipuncture. The serum should be separated from the cells after clot formation by centrifugation. For plasma samples, blood should be collected into a container with an anticoagulant and then centrifuged. Care should be taken to minimize hemolysis, excessive hemolysis can impact your results. Assay immediately or aliquot and store samples at -20°C. Avoid repeated freeze thaw cycles.

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Additional Information

Assay Background

Antithrombin III is a small glycoprotein produced by the liver. It is a major inhibitor of the coagulation cascade and inhibits virtually all of the coagulation enzymes, including Thrombin. Deficiency of Antithrombin III, either genetic or acquired can lead to Thromboembolic disease.

Typical Standard Curve

Rat Antithrombin III ELISA


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