VIP ELISA Assay Kit
The VIP ELISA Assay Kit is For Research Use Only
Size: 1×96 wells
Sensitivity: 0.12 ng/ml
Dynamic Range: 0.04-25 ng/mL
Incubation Time: 4.5 hours
Sample Type: Plasma
Sample Size: 50 µL
Alternative Names: Vasoactive Intestinal Peptide, Multispecies VIP
SAMPLE COLLECTION & STORAGE INFORMATION
The sample collection and storage conditions listed below are intended as general guidelines. Sample stability has not been evaluated.
Serum- Use a serum separator tube (SST) and allow samples to clot for 30 minutes before centrifugation for 15 minutes at 1600 x g at 4°C. Collect serum and assay immediately or aliquot and store samples at ≤ -80 °C up to 1 month. Avoid repeated freeze-thaw cycles.
Plasma – Collect plasma using EDTA as an anticoagulant. Centrifuge for 15 minutes at 4°C at 1000 x g within 30 minutes of collection. Assay immediately or aliquot and store samples at ≤ -80 °C up to 1 month. Avoid repeated freeze thaw cycles.
VIP (vasoactive intestinal peptide) belongs to the glucagon family. It stimulates myocardial contractility, causes vasodilation, increases glycogenolysis, lowers arterial blood pressure and relaxes the smooth muscle of trachea, stomach and gall bladder. The protein also acts as an antimicrobial peptide with antibacterial and antifungal activity. Alternative splicing occurs at this locus and two transcript variants encoding distinct isoforms have been identified.
VIP causes vasodilation, lowers arterial blood pressure, stimulates myocardial contractility, increases glycogenolysis and relaxes the smooth muscle of the trachea, stomach and gall bladder. PHM and PHV also cause vasodilation. PHM-27 is a potent agonist of the calcitonin receptor CALCR, with similar efficacy as calcitonin.