Vero HCP ELISA Assay Kit
The Vero HCP ELISA Assay Kit is For Research Use Only
Size: 1 x 96 wells
Sensitivity: 0.20 ng/ml
Calibrator Range: 0.312 – 20 ng/ml
Sample Size: 100 uL
Sample Type: Biological Products
Incubation Time: 3 hours 20 minutes
Assay Background
A variety of proteins which are used as therapeutic agents in humans and animals are produced through recombinant expression in VERO cells. The manufacturing and purification process of these products tends to leave the potential for contamination by Host Cell Proteins (HCPs) from VERO cells, which may result in adverse
toxic or immunological reactions that ultimately affect the efficacy of the therapeutic agent. The simple, objective and semi-quantitative ELISA is a highly sensitive method that aids in purification process development, process control, quality control and product release testing optimally.
Assay Principle
The method employs sandwich ELISA technique. Monoclonal antibodies are pre-coated onto microwells. Samples and standards are pipetted into microwells and Vero HCP present in the sample are bound by the antibodies. Biotin labeled antibody is added and followed by Streptavidin-HRP is pipetted and incubated to form a complex. After washing microwells in order to remove any non-specific binding, the substrate solution (TMB) is added to microwells and color develops proportionally to the amount of Vero HCP in the sample. Color development is then stopped by addition of stop solution. Absorbance is measured at 450 nm.
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