Human (Leu27) IGF-II
The Eagle Bioscience’s Human (Leu27) IGF-II is for research use only and not to be used for diagnostic procedures.
Product Specifications
Source:
IGF-II is produced in E.coli using a patented expression system.
Description:
Human (Leu27) insulin-like Growth Factor-II ([Leu27] IGF-II) is an analogue of human IGF-II which has a Leucine substitution in place of Tyrosine at position 27 of the human IGF-II sequence. (Leu27) IGF-II has normal binding to acid-stripped human serum binding proteins but has a 10-20 fold reduction in binding to the type 1 IGF receptor compared to IGF-II. It is a useful analogue for studying the effects of IGF-II through non-type 1 receptor binding mechanisms.
- Purity: >90 % (as determined by HPLC analysis)
- Quantity: 100 µg
- Molecular Weight: 7420 daltons – confirmed by Mass Spectrometry
- N-terminal sequence analysis of recombinant product: Analysis of 5 residues
- Biological Activity: IGF type 1 receptor binding assay (ED50 > 175 ng/ml) IGF type 2 receptor binding assay (ED50 < 370 ng/ml) IGF binding protein assay: ED50 < 10 ng/ml)
- Endotoxin (LAL): <0.1 EU/ug
- Storage/Stability: At least 2 years at 2-4°C (lyophilized)
- Appearance: White Powder
- State: Dried from 0.1 M acetic acid under dry nitrogen at a slight vacuum (-25kPa)
Related Products
IGF-II ELISA Assay Kit
Human Biotin Pro IGF-2
Biotinylated Human Des IGF-2
If you have any questions about this product or any of our other offerings, contact us here.
