HEK HCP ELISA Assay Kit
The HEK HCP ELISA Assay Kit is For Research Use Only
Size: 1 x 96 wells
Calibrator Range: 0.313 – 20 ng/mL
Sensitivity: 0.188 ng/ mL
Sample Size: 100 uL
Sample Type: serum, plasma, and other biological samples
Incubation Time: 3 hours 10 minutes
Controls Not Included
Assay Background
This ELISA kit is used for assessing the specific biomarker in samples analytes which may be serum, plasma and cell culture supernatant as validated with the kit. The kit employs a sandwich ELISA technique which leads to a higher specificity and increased sensitivity compared to conventional competitive ELISA kits which employ only one antibody. Double antibodies are used in this kit.
Assay Principle
The method employs sandwich ELISA technique. Monoclonal antibodies are pre-coated onto microwells. Samples and standards are pipetted into microwells and Human HEK HCP present in the sample are bound by the antibodies. Biotin labeled antibody is added and followed by Streptavidin-HRP is pipetted and incubated to form a complex. After washing microwells in order to remove any non-specific binding, the substrate solution (TMB) is added to microwells and color develops proportionally to the amount of Human HEK HCP in the sample. Color development is then stopped by addition of stop solution. Absorbance is measured at 450 nm.
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