SP 2/0 HCP ELISA Assay Kit


The SP 2/0 HCP ELISA Assay Kit is intended in determining the presence of SP2/0 Host Cell Proteins contamination in various products that are manufactured through recombinant expression in SP2/0 cells. The kit has been validated successfully for testing of in process and final product HCPs in variety of products regardless of growth and purification process. The SP 2/0 HCP ELISA Assay Kit is for research use only and not to be used for diagnostic procedures.

SP 2/0 HCP ELISA Assay Kit

The SP 2/0 HCP ELISA Assay Kit is For Research Use Only

Size: 1 x 96 wells
Calibrator Range: 0 – 300 ng/mL
Sensitivity: 1 ng/mL
Sample Size: 100 uL
Sample Type: Cell Culture Supernatant and other Biological Preparations
Incubation Time: 2 hours

Controls Not Included

Assay Background

Recombinant expression by the hybridoma cell line SP2/0 is a relatively simple and cost effective method for production of monoclonal antibodies intended for use as therapeutic agents in humans.

Assay Principle

This assay is based on the Sandwich ELISA procedure. Microwells are coated with anti- SP2/0 antibody. Addition of Anti- SP2/0:HRP Conjugate and standards or samples containing SP2/0 HCPs will form a immune complex with the coated anti- SP2/0 antibody in microwell. A washing cycle will remove free Anti- SP2/0:HRP Conjugate from the reaction well. Addition of TMB Substrate will produce blue color in microwells containing immune complex. Addition of stop solution will terminate the enzymatic reaction and blue color will be converted to yellow color which is directly proportional to the concentration of SP2/0 HCPs present and absorbance value is measured at 450nm.

Related Products:

Pichia Pastoris HCP ELISA Assay Kit
E.coli HCP ELISA Assay Kit


Product Manual

Please note: All documents above are for reference use only and should not be used in place of the documents included with this physical product. If digital copies are needed, please contact us.

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