Urine Creatinine Detection Kit
The Urine Creatinine Detection Kit is For Research Use Only
Size: 2×96 wells
Sensitivity: 0.019 mg/dl
Dynamic Range: 0.3125- 20 mg/dl
Incubation Time: 30 minutes
Sample Type: Urine
Sample Size: 50 μL
Product manufactured in Canada by StressMarq.
Creatinine (2-amino-1-methyl-5H-imadazol-4-one) is a metabolite of phospho-creatine (p-creatine), a molecule used as a store for high-energy phosphate that can be utilized by tissues for the production of ATP. Creatine either comes from the diet or synthesized from the amino acids arginine, glycine, and methionine. This occurs in the kidneys and liver, although other organ systems may be involved and species-specific differences may exist2. Creatine and p-creatine are converted non-enzymatically to the metabolite creatinine, which diffuses into the blood and is excreted by the kidneys. In vivo, this conversion appears to be irreversible and in vitro it is favored by higher temperatures and lower pH. Creatinine forms spontaneously from p-creatine. Under normal conditions, its formation occurs at a rate that is relatively constant and as intra-individual variation is <15% from day to day, creatinine is a useful tool for normalizing the levels of other molecules found in urine. Additionally altered creatinine levels may be associated with other conditions that result in decreased renal blood flow such as diabetes and cardiovascular disease.
Sample Types Validated:
Human, Monkey, Dog, and Rat Urine
This assay has been validated for human, rat, dog and monkey urine samples. Urine samples containing visible protein or particulates should be centrifuged or filtered prior to using. Mouse urine samples are not compatible with the use of this assay to determine GFR as over half of murine urinary creatinine is from renal secretion rather than filtration.