Corticosterone EIA Kit


The Corticosterone EIA Kit is designed to quantitatively measure Corticosterone present in serum, plasma, urine, extracted dried fecal samples, and tissue culture media samples.

SKU: SKT-205-96 Categories: , ,

Corticosterone EIA Kit

The Corticosterone EIA Kit is For Research Use Only

Size: 1×96 wells
Sensitivity: 18.6 pg/ml
Dynamic Range: 78.125 – 10,000 pg/ml
Incubation Time: 1.5 hours
Sample Type: Dried Fecal Samples, EDTA Plasma, Heparin Plasma, Serum, Tissue Culture Media, Urine
Sample Size: 50 μL

Product manufactured in Canada by StressMarq.

Assay Principle

This kit measures total corticosterone in serum and plasma and in extracted fecal samples. A corticosterone stock solution is provided to generate a standard curve for the assay and all samples should be read off the standard curve. We provide protocols on page 8 to prepare assay standards from 5,000 to 78.125 pg/mL or from 10,000 to 78.125 pg/mL. Please choose the standard range that fits your sample concentrations most appropriately. Standards or diluted samples are pipetted into a clear microtiter plate coated with an antibody to capture sheep antibodies. A corticosterone-peroxidase conjugate is added to the standards and samples in the wells. The binding reaction is initiated by the addition of a polyclonal antibody to corticosterone to each well. After an hour incubation the plate is washed and substrate is added. The substrate reacts with the bound corticosterone-peroxidase conjugate. After a short incubation, the reaction is stopped and the intensity of the generated color is detected in a microtiter plate reader capable of measuring 450nm wavelength. The concentration of the corticosterone in the sample is calculated, after making suitable correction for the dilution of the sample, using software available with most plate readers.

Related Products

Urinary Cortisol ELISA Assay
Cortisol LIA Assay Kit

Additional Information

Assay Background

Corticosterone (C21H30O4, Kendall’s Compound ‘B’) is a glucocorticoid secreted by the cortex of the adrenal gland. Corticosterone is produced in response to stimulation of the adrenal cortex by ACTH and is the precursor of aldosterone. Corticosterone is a major indicator of stress and is the major stress steroid produced in non-human mammals. Studies involving corticosterone and levels of stress include impairment of long term memory retrieval (1), chronic corticosterone elevation due to dietary restrictions (2) and in response to burn injuries (3). In addition to stress levels, corticosterone is believed to play a decisive role in sleep-wake patterns (4,5).

Typical Standard Curve

Package Inserts

Please note: All documents above are for reference use only and should not be used in place of the documents included with this physical product. If digital copies are needed, please contact us.

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