Creatinine Serum Detection Kit
The Creatinine Serum Detection Kit is For Research Use Only
Size: 2×96 wells
Sensitivity: 0.081 mg/dl
Dynamic Range: 0.5 – 4 mg/dl
Incubation Time: 30 minutes
Sample Type: Plasma, Serum
Sample Size: 25 μL
Product manufactured in Canada by StressMarq.
Creatinine (2-amino-1-methyl-5H-imadazol-4-one) is a metabolite of phosphocreatine (p-creatine), a molecule used as a store for high-energy phosphate that can be utilized by tissues for the production of ATP. Creatine either comes from the diet or synthesized from the amino acids arginine, glycine, and methionine. This occurs in the kidneys and liver, although other organ systems may be involved and species-specific differences may exist. Creatine and p-creatine are converted non-enzymatically to the metabolite creatinine, which diffuses into the blood and is excreted by the kidneys. In vivo , this conversion appears to be irreversible and in vitro it is favored by higher temperatures and lower pH2. Creatinine forms spontaneously from p-creatine. Under normal conditions, its formation occurs at a rate that is relatively constant and as intra- individual variation is <15% from day to day, creatinine is a useful tool for normalizing the levels of other molecules found in urine. Additionally altered creatinine levels may be associated with other conditions that result in decreased renal blood flow such as diabetes and cardiovascular disease.
Sample Types Validated:
Mammalian Serum and Plasma
This assay has been validated for human, mouse, rabbit, rat and sheep serum and EDTA and heparin plasma samples. The end user should evaluate recoveries of creatinine in other plasma and serum samples being used. Hemolyzed or lipemic samples should not be
used with this kit. Hemolyzed samples have shown a decrease in creatinine concentration with increasing hemoglobin, whereas lipemic samples have been shown to yield artificially high creatinine concentrations.