Creatinine Serum Detection Kit

$350.00

The Creatinine Serum Detection Kit is designed to quantitatively measure creatinine present in serum samples.

SKU: SKT-217-192 Categories: , ,

Creatinine Serum Detection Kit

The Creatinine Serum Detection Kit is For Research Use Only

Size: 2×96 wells
Sensitivity: 0.081 mg/dl
Dynamic Range: 0.5 – 4 mg/dl
Incubation Time: 30 minutes
Sample Type: Plasma, Serum
Sample Size: 25 μL

Product manufactured in Canada by StressMarq.


Assay Background

Creatinine (2-amino-1-methyl-5H-imadazol-4-one) is a metabolite of phosphocreatine (p-creatine), a molecule used as a store for high-energy phosphate that can be utilized by tissues for the production of ATP. Creatine either comes from the diet or synthesized from the amino acids arginine, glycine, and methionine. This occurs in the kidneys and liver, although other organ systems may be involved and species-specific differences may exist. Creatine and p-creatine are converted non-enzymatically to the metabolite creatinine, which diffuses into the blood and is excreted by the kidneys. In vivo , this conversion appears to be irreversible and in vitro it is favored by higher temperatures and lower pH2. Creatinine forms spontaneously from p-creatine. Under normal conditions, its formation occurs at a rate that is relatively constant and as intra- individual variation is <15% from day to day, creatinine is a useful tool for normalizing the levels of other molecules found in urine. Additionally altered creatinine levels may be associated with other conditions that result in decreased renal blood flow such as diabetes and cardiovascular disease.


Sample Types
Sample Types Validated:
Mammalian Serum and Plasma
This assay has been validated for human, mouse, rabbit, rat and sheep serum and EDTA and heparin plasma samples. The end user should evaluate recoveries of creatinine in other plasma and serum samples being used. Hemolyzed or lipemic samples should not be
used with this kit. Hemolyzed samples have shown a decrease in creatinine concentration with increasing hemoglobin, whereas lipemic samples have been shown to yield artificially high creatinine concentrations.

Related Products

Urine Creatinine Detection Kit
Hydrogen Peroxide Detection Kit
Blood Urea Nitrogen Detection Kit

Additional Information

Assay Principle


A creatinine standard, calibrated to a NIST creatinine standard, is provided to generate a standard curve for the assay and all samples should be read off the standard curve. Standards or samples are pipetted into a clear microtiter plate. An assay diluent is added to all standards, controls and samples. The color generating reaction is initiated with the StressXpress® Creatinine Reagent, which is pipetted into each well. The assay utilizes a kinetic absorbance method to overcome interference by colored compounds in serum. The absorbance of the colored product is read after 1 minute in a microtiter plate reader capable of measuring 490nm wavelength. At 30 minutes the optical density is read again. The concentration of creatinine is calculated using the delta of the optical density readings at 30 and 1 minute compared to the curve generated from the standards, or by using the Excel worksheet available for free download at our web site. The Jaffe reaction used in this kit has been modified to read creatinine levels in serum,8.

Typical Standard Curve


Creatinine Serum Detection Kit

Manual

Product Manual