Free Testosterone LIA Assay Kit
The Free Testosterone LIA Assay Kit is For Research Use Only
Size: 1×96 wells
Sensitivity: 0.17 pg/mL
Dynamic Range: 0.25–125 pg/mL
Incubation Time: 90 minutes
Sample Type: Serum
Sample Size: 25 μL
The principle of the Free Testosterone LIA Kit chemiluminescence immunoassay (LIA) test follows the typical competitive binding scenario. Competition occurs between an unlabelled antigen (present in standards, control and patient samples) and an enzyme-labelled antigen (conjugate) for a limited number of antibody binding sites on the microplate. The washing and decanting procedures remove unbound materials. After the washing step, the luminescence substrate solution is added. The relative luminescence units (RLUs) are measured on a microtiter plate luminometer. The RLU values are inversely proportional to the concentration of free testosterone in the sample. A set of calibrators are used to plot a standard curve from which the amount of free testosterone in patient samples and controls can be directly read. The labelled testosterone (conjugate) employed in this assay system has shown no binding properties towards SHBG and human serum albumin. A highly specific rabbit anti-testosterone polyclonal antibody at a low binding capacity (Keq x concentration) is used to keep minimum disturbances of the testosterone-protein equilibrium. The other components in the test system are also optimized in order to not alter the original free testosterone concentration.
SPECIMEN COLLECTION AND STORAGE
Approximately 0.2 mL of serum is required per duplicate determination. Collect 4–5 mL of blood into an appropriately labelled tube and allow it to clot. Centrifuge and carefully remove the serum layer. Store at 4°C for up to 24 hours or at -10°C or lower if the analyses are to be done at a later date. Consider all human specimens as possible biohazardous materials and take appropriate precautions when handling.