Total Prekallikrein ELISA Assay
The Total Prekallikrein ELISA Assay is For Research Use Only
Size: 1×96 wells
Dynamic Range: 20-10000 ng/ml
Incubation Time: 2 hours
Sample Type: Plasma
Sample Size: 100 µL
Alternative Name: Human Prekallikrein total antigen ELISA
SAMPLE COLLECTION & STORAGE INFORMATION
The sample collection and storage conditions listed below are intended as general guidelines. Sample stability has not been evaluated.
Plasma: Collect plasma using EDTA or citrate as an anticoagulant. Centrifuge for 15 minutes at 1000xg within 30 minutes of collection. Assay immediately or aliquot and store at ≤ -20°C. Avoid repeated freeze-thaw cycles.
The Prekallikrein ELISA employs the sandwich enzyme immunoassay technique for the detection of Human Prekallikrein (total) in human plasma samples. Human Prekallikrein will bind to the capture antibody coated on the microtiter plate. After appropriate washing steps, anti-Human Prekallikrein primary antibody binds to the captured protein. Following a washing to remove unbound substances, secondary antibody conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After washing away any unbound antibody-enzyme reagent, a substrate solution (TMB) is added to the wells and color develops in proportion to the amount of Prekallikrein bound in the initial step. The color development is stopped by the addition of acid and the intensity of the color is measured at a wavelength of 450nm ±2nm. The concentration of Prekallikrein in the sample is then determined by comparing the O.D of samples to the standard curve.