Tissue Inhibitor of Metalloproteinases-1 (TIMP-1) ELISA Assay Kit

$560.00

The Tissue Inhibitor of Metalloproteinases-1 (TIMP-1) ELISA Assay kit is designed for quantification of human TIMP-1 in serum, plasma and cell culture supernate samples. The Eagle Biosciences Tissue Inhibitor of Metalloproteinases-1 (TIMP-1) ELISA Assay kit is for research use only and not to be used for diagnostic purposes.

SKU: TMP31-K01 Categories: , ,

Tissue Inhibitor of Metalloproteinases-1 (TIMP-1) ELISA Assay Kit

The Tissue Inhibitor of Metalloproteinases-1 (TIMP-1) ELISA Assay Kit is For Research Use Only

Size: 1×96 wells
Sensitivity: 31.2pg/ml
Standard Range: 31.2pg/ml – 2000pg/ml
Incubation Time: 3 hrs 35 mins
Sample Type: Serum, plasma, and cell culture supernates
Sample Size: 100 µl


Assay Principle

Th Tissue Inhibitor of Metalloproteinases-1 (TIMP-1) assay is a quantitative sandwich ELISA. The immunoplate is pre-coated with a monoclonal antibody specific for human TIMP-1. Standards and samples are pipetted into the wells and any human TIMP-1 present is bound by the immobilized antibody. After washing away any unbound substances, a biotin labelled polyclonal antibody specific for human TIMP-1 is added to the wells. After wash step to remove any unbound reagents, streptavidin-HRP conjugate (STP-HRP) is added. After the last wash step, an HRP substrate solution is added and colour develops in proportion to the amount of human TSP-2 bound initially. The assay is stopped and the optical density of the wells determined using a microplate reader. Since the increases in absorbance are directly proportional to the amount of captured human TIMP-1, the unknown sample concentration can be interpolated from a reference curve included in each assay.


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Additional Information

Assay Background


Tissue inhibitor of metalloproteinases-1(TIMP-1) plays a crucial role in extracellular matrix (ECM) composition and wound healing. It has been found to be strongly upregulated in liver tissue and serum during hepatic fibrogenesis in patients with liver disease and in animal models of hepatic fibrogenesis.

Typical Standard Curve


Tissue Inhibitor of Metalloproteinases-1 ELISA Standard Curve

Documents

Product Manual


Product Citations