Thyroid Stimulating Hormone LIA
The Thyroid Stimulating Hormone LIA is For Research Use Only
Size: 1×96 wells
Sensitivity: 0.13 μIU/mL
Dynamic Range: 0.15–30 μIU/mL
Incubation Time: 90 minutes
Sample Type: Serum
Sample Size: 50 μL
Alternative Names: TSH LIA, Human Thyroid Stimulating Hormone LIA, Human TSH LIA
Controls Included
Assay Principle
The principle of this chemiluminescence immunoassay test follows a typical one-step capture or ‘sandwich’ type assay. The assay makes use of two highly specific monoclonal antibodies: A monoclonal antibody specific for TSH is immobilized onto the microplate and another monoclonal antibody specific for a different region of TSH is conjugated to horse radish peroxidase (HRP). TSH from the sample and standards are allowed to bind simultaneously to the plate and to the HRP conjugate. The washing and decanting steps remove any unbound HRP conjugate. After the washing step, the luminescence substrate is added and the relative luminescence units (RLUs) are measured in a microtiter plate luminometer. The RLUs formed by the enzymatic reaction are directly proportional to the concentration of TSH in the samples. A set of standards is used to plot a standard curve from which the concentration of TSH in patient samples and controls are read.
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