Testosterone Saliva LIA Assay


The Testosterone Saliva LIA Assay Kit is for the direct quantitative determination of testosterone in human saliva by a chemiluminescence immunoassay (LIA). The Eagle Biosciences Testosterone Saliva LIA assay kit is for research use only and not to be used in diagnostic procedures.

SKU: TST32-L01 Categories: , ,

Testosterone Saliva LIA Assay

The Testosterone Saliva LIA Assay is For Research Use Only

Size: 1×96 wells
Sensitivity: 1 pg/mL
Dynamic Range: 2–800 pg/mL
Incubation Time: 120 minutes
Sample Type: Saliva
Sample Size: 100 μL
Controls Included

Assay Principle for Testosterone Saliva LIA

The principle of the Testosterone Saliva chemiluminescence immunoassay (LIA) test follows a two-step competitive binding scenario. Competition occurs between an unlabelled antigen (present in standards, controls and patient samples) and a biotin-labelled antigen (conjugate) for a limited number of antibody binding sites on the microplate. After washing the streptavidin-horseradish peroxidase conjugate is incubated and bound to any bound biotinylated testosterone. The washing and decanting procedures remove unbound materials. After the second washing step, the luminescence substrate solution is added. The relative luminescence units (RLUs) are measured on a microtiter plate luminometer. The RLU values are inversely proportional to the concentration of testosterone in the sample. A set of calibrators is used to plot a standard curve from which the amount of testosterone in patient samples and controls can be directly read.

Approximately 1 mL of saliva is required per duplicate determination. Collect 2–3 mL of saliva into a clean glass tube without force or inducement and before eating, drinking or brushing the teeth. Simply rinse the mouth with water before collection and wait a few minutes to start. Do not use blood-contaminated specimens.
Human fluids that may be used in the preparation of the standards and controls has been tested and found to be nonreactive for Hepatitis B surface antigen and has also been tested for the presence of antibodies to HCV and Human Immunodeficiency Virus (HIV) and found to be negative. No test method however, can offer complete assurance that HIV, HCV and Hepatitis B virus or any infectious agents are absent. The reagents should be considered a potential biohazard and handled with the same precautions as applied to any blood specimen.

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Additional Information

Assay Background

Testosterone is a C-19 steroid secreted from the testis and the adrenal cortex in men and from the adrenal cortex and ovary in women. Testosterone is also produced by peripheral tissues from androstenedione, which is of little physiological significance in men, however in women about half of circulating testosterone is derived from this origin. The action of testosterone is both androgenic and anabolic. Testosterone measurements are used mainly for clinical evaluation of hypogonadism in males and hyperandrogenic states in females. Most of the circulating testosterone is bound to three proteins: sex hormone binding globulin (44–78%), albumin (20–54%) and cortisol binding globulin (small amount). Only about 2–3% of the total circulating testosterone remains unbound or in the free form. Only the free portion (or the non-SHBG bound fraction) of the circulating testosterone is thought to be available to tissues where it exerts its biological actions. The salivary hormone assays are advocated for their noninvasive, easy sample collection method. Salivary testosterone is of great clinical value for it represents a filtered fraction of plasma testosterone and is independent of flow rate. Many studies have suggested that salivary testosterone correlates well with either free or non-SHBG bound testosterone.

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