Testosterone Saliva LIA Assay
The Testosterone Saliva LIA Assay is For Research Use Only
Size: 1×96 wells
Sensitivity: 1 pg/mL
Dynamic Range: 2–800 pg/mL
Incubation Time: 120 minutes
Sample Type: Saliva
Sample Size: 100 μL
Assay Principle for Testosterone Saliva LIA
The principle of the Testosterone Saliva chemiluminescence immunoassay (LIA) test follows a two-step competitive binding scenario. Competition occurs between an unlabelled antigen (present in standards, controls and patient samples) and a biotin-labelled antigen (conjugate) for a limited number of antibody binding sites on the microplate. After washing the streptavidin-horseradish peroxidase conjugate is incubated and bound to any bound biotinylated testosterone. The washing and decanting procedures remove unbound materials. After the second washing step, the luminescence substrate solution is added. The relative luminescence units (RLUs) are measured on a microtiter plate luminometer. The RLU values are inversely proportional to the concentration of testosterone in the sample. A set of calibrators is used to plot a standard curve from which the amount of testosterone in patient samples and controls can be directly read.
SPECIMEN COLLECTION AND STORAGE
Approximately 1 mL of saliva is required per duplicate determination. Collect 2–3 mL of saliva into a clean glass tube without force or inducement and before eating, drinking or brushing the teeth. Simply rinse the mouth with water before collection and wait a few minutes to start. Do not use blood-contaminated specimens.
POTENTIAL BIOHAZARDOUS MATERIAL
Human fluids that may be used in the preparation of the standards and controls has been tested and found to be nonreactive for Hepatitis B surface antigen and has also been tested for the presence of antibodies to HCV and Human Immunodeficiency Virus (HIV) and found to be negative. No test method however, can offer complete assurance that HIV, HCV and Hepatitis B virus or any infectious agents are absent. The reagents should be considered a potential biohazard and handled with the same precautions as applied to any blood specimen.