Thrombospondin 2 ELISA Kit
Thrombospondin 2 ELISA Kit is For Research Use Only
Size: 1×96 wells
Sensitivity: 0.156ng/ml
Standard Range: 0.156ng/ml-10ng/ml
Incubation Time: 3 hours 35 minutes
Sample Type: Serum and plasma
Sample Size: 100 µl
Alternative Names: TSP-2
Assay Principle
The Thrombospondin 2 (TSP-2) assay is a quantitative sandwich ELISA. The immunoplate is pre-coated with a monoclonal antibody specific for human TSP-2. Standards and samples are pipetted into the wells and any human TSP-2 present is bound by the immobilized antibody. After washing away any unbound substances, a biotin labeled polyclonal antibody specific for human TSP-2 is added to the wells. After wash step to remove any unbound reagents, streptavidin-HRP conjugate (STP-HRP) is added. After the last wash step, an HRP substrate solution is added and colour develops in proportion to the amount of human TSP-2 bound initially. The assay is stopped and the optical density of the wells determined using a microplate reader. Since the increases in absorbance are directly proportional to the amount of captured human TSP-2, the unknown sample concentration can be interpolated from a reference curve included in each assay kit.
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