Estriol Saliva LIA Assay Kit

$190.00

The Estriol Saliva LIA Assay Kit is for the direct quantitative determination of estriol in human saliva by a chemiluminescence immunoassay (LIA). The Eagle Biosciences Estriol Saliva LIA Assay kit is for research use only and not to be used in diagnostic procedures.

SKU: ESL32-L01 Categories: , ,

Estriol Saliva LIA Assay Kit

The Estriol Saliva LIA Assay Kit is For Research Use Only

Size: 1×96 wells
Sensitivity: 0.03 ng/mL
Dynamic Range: 0.03–30 ng/mL
Incubation Time: 95 minutes
Sample Type: Saliva
Sample Size: 50 μL
Controls Included


Assay Background for Estriol Saliva LIA Assay Kit

During pregnancy the determination of estriol is used for monitoring fetal well being. Therefore, during pregnancy the estriol level can detect abnormalities in the development of the fetus. As pregnancy progresses in time the concentration of Estriol also increases. The unconjugated estriol saliva determination is of great clinical importance to follow the progress of pregnancy since the level of salivary unconjugated estriol reflects the unconjugated estriol concentration in serum, which reflects the biologically active fraction. The collection of saliva has advantages compared to urine and serum since it is a non-invasive procedure which can be easily collected at home and stored frozen. Due to the low concentration of unconjugated estriol in saliva we have developed an assay system using a chemiluminescent immunoassay (LIA). The Estriol Saliva LIA Kit assay is very sensitive (0.03 ng/mL), precise and accurate along with a short incubation time of 75 minutes at RT° on a shaker.


SPECIMEN COLLECTION AND STORAGE
Approximately 1 mL of saliva is required per duplicate determination. Collect 4–5 mL of saliva into a clean glass tube (Salivette by Sarstedt may be used) without force or inducement and before eating, drinking or brushing the teeth. Simply rinse the mouth with water before collection. Do not use blood-contaminated specimens. Store samples at 4°C for up to 24 hours or at -10°C or lower if the analyses are to be done at a later date. Consider all human specimens as possible biohazardous materials and take appropriate precautions when handling.


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Additional Information

Assay Principle


The principle of the following chemiluminescence immunoassay (LIA) test follows the typical competitive binding scenario. Competition occurs between an unlabelled antigen (present in standards, control and patient samples) and an enzyme-labelled antigen (conjugate) for a limited number of antibody binding sites on the microplate. The washing and decanting procedures remove unbound materials. After the washing step, the luminescence substrate solution is added. The relative luminescence units (RLUs) are measured on a microtiter plate luminometer. The RLU values are inversely proportional to the concentration of estriol in the sample. A set of calibrators are used to plot a standard curve from which the amount of Estriol in patient samples and controls can be directly read.

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