Testosterone Saliva ELISA Assay
The Testosterone Saliva ELISA Assay is For Research Use Only
Size: 1×96 wells
Sensitivity: 1 pg/mL
Dynamic Range: 2–800 pg/mL
Incubation Time: 105 minutes
Sample Type: Saliva
Sample Size: 100 µl
The principle of the following enzyme immunoassay test follows a two-step competitive binding scenario. Competition occurs between an unlabeled antigen (present in standards, controls and patient samples) and a biotin-labelled antigen (conjugate) for a limited number of antibody binding sites on the microplate. In the second step, the streptavidin-horseradish peroxidase conjugate binds to any bound biotinylated testosterone. The washing and decanting procedures remove unbound materials. After the last washing step, the enzyme substrate is added. The enzymatic reaction is terminated by addition of the stopping solution. The absorbance is measured on a microtiter plate reader. The intensity of the color formed is inversely proportional to the concentration of testosterone in the sample. A set of standards is used to plot a standard curve from which the amount of testosterone in patient samples and controls can be directly read.
SPECIMEN COLLECTION AND STORAGE
Approximately 1 mL of saliva is required per duplicate determination. Collect 2–3 mL of saliva into a clean glass tube without force or inducement and before eating, drinking or brushing the teeth. Simply rinse the mouth with water before collection. Do not use blood-contaminated specimens. Store samples at 4°C for up to 24 hours or at -10°C or lower if the analyses are to be done at a later date. Consider all human specimens as possible biohazardous materials and take appropriate precautions when handling.
POTENTIAL BIOHAZARDOUS MATERIAL
Human serum that may be used in the preparation of the standards and controls has been tested and found to be nonreactive for Hepatitis B surface antigen and has also been tested for the presence of antibodies to HCV and Human Immunodeficiency Virus (HIV) and found to be negative. No test method however, can offer complete assurance that HIV, HCV and Hepatitis B virus or any infectious agents are absent. The reagents should be considered a potential biohazard and handled with the same precautions as applied to any blood specimen.