Bovine CRP ELISA Assay Kit
The Bovine CRP ELISA Assay Kit is For Research Use Only
Size: 1×96 wells
Dynamic Range: 1.56 ng/ml – 100 ng/ml
Incubation Time: 1 hour 40 minutes
Sample Type: Milk, Plasma, Serum
Sample Size: 100 μL
Alternative Names: CRP, C-Reactive Protein
Assay Principle
The principle of the double antibody sandwich ELISA is represented in Figure 1. In this assay the c-reactive protein (CRP) present in samples reacts with the anti-CRP antibodies, which have been adsorbed to the surface of polystyrene microtiter wells. After the removal of unbound proteins by washing, anti-CRP antibodies conjugated with horseradish peroxidase (HRP) are added. These enzyme-labeled antibodies form complexes with the previously bound CRP. Following another washing step, the enzyme bound to the immunosorbent is assayed by the addition of a chromogenic substrate, 3,3’,5,5’-tetramethylbenzidine (TMB). The quantity of bound enzyme varies directly with the concentration of CRP in the sample tested; thus, the absorbance, at 450 nm, is a measure of the concentration of CRP in the test sample. The quantity of CRP in the test sample can be interpolated from the standard curve constructed from the standards, and corrected for sample dilution.
Specimen Collection
- Serum samples – Blood should be collected by venipuncture. The serum should be separated from the cells after clot formation by centrifugation. Remove serum and assay immediately or aliquot and store samples at –80oC (preferably) or -20oC. Avoid repeated freeze-thaw cycles.
- Plasma samples – Blood should be collected into a container with an anticoagulant and then centrifuged. Assay immediately or aliquot and store samples at –80oC (preferably) or -20oC. Avoid repeated freeze-thaw cycles.
- Urine samples – Collect mid-stream using sterile or clean urine collector. Centrifuge to remove cell debris. Assay immediately or aliquot and store samples at –80oC (preferably) or -20oC. Avoid repeated freeze-thaw cycles.
- Known interfering substances – Azide and thimerosal at concentrations higher than 0.1% inhibits the enzyme reaction.
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