Testosterone Saliva ELISA Assay Kit


The Eagle Biosciences Testosterone Saliva ELISA Assay Kit (enzyme-linked immunoassay kit) is intended for the direct quantitative determination of testosterone in human saliva. The Testosterone Saliva ELISA Assay Kit is for research use only and not to be used in diagnostic procedures.

SKU: TSS32-K01 Categories: , ,

Testosterone Saliva ELISA Assay Kit

For Research Use Only

Size: 1×96 wells
Sensitivity: 1 pg/mL
Dynamic Range: 2–800 pg/mL
Incubation Time: 105 minutes
Sample Type: Saliva
Sample Size: 100 µl

Controls Included

Additional Information

Assay Background

Testosterone is a C-19 steroid secreted from the testis and the adrenal cortex in men and from the adrenal cortex and ovary in women. Testosterone is also produced by peripheral tissues from androstenedione, which is of little physiological significance in men, however in women about half of circulating testosterone is derived from this origin. The action of testosterone is both androgenic or anabolic. Testosterone measurements are used mainly for clinical evaluation of hypogonadism in males and hyperandrogenic states in females. Most of the circulating testosterone is bound to three proteins: sex hormone binding globulin (44–78%), albumin (20–54%) and cortisol binding globulin (small amount). Only about 2–3% of the total circulating testosterone remains unbound or in the free form. Only the free portion (or the non-SHBG bound fraction) of the circulating testosterone is thought to be available to tissues where it exerts its biological actions. The salivary hormone assays are advocated for their noninvasive, easy sample collection method. Salivary testosterone is of great clinical value for it represents a filtered fraction of plasma testosterone and is independent of flow rate. Many studies have suggested that salivary testosterone correlates well with either free or non-SHBG bound testosterone.

Assay Principle

The principle of the following enzyme immunoassay test follows a two-step competitive binding scenario. Competition occurs between an unlabeled antigen (present in standards, controls and patient samples) and a biotin-labelled antigen (conjugate) for a limited number of antibody binding sites on the microplate. In the second step, the streptavidin-horseradish peroxidase conjugate binds to any bound biotinylated testosterone. The washing and decanting procedures remove unbound materials. After the last washing step, the enzyme substrate is added. The enzymatic reaction is terminated by addition of the stopping solution. The absorbance is measured on a microtiter plate reader. The intensity of the color formed is inversely proportional to the concentration of testosterone in the sample. A set of standards is used to plot a standard curve from which the amount of testosterone in patient samples and controls can be directly read.


Product Manual